AI Article Synopsis
- Human cytomegalovirus (CMV) significantly affects individuals with weakened immune systems, yet no effective vaccine exists.
- Identifying target proteins and T-cell epitopes is essential for creating a successful vaccine and for new techniques involving T-cell therapy.
- The study introduces a streamlined SPOT synthesis method to create and test all potential CD8 T-cell epitopes from the CMV proteome, leading to the discovery of many known and novel epitopes after testing over 9,000 peptides.
Article Abstract
Human cytomegalovirus (CMV) is a major cause of morbidity in immunocompromised individuals. However, no efficient vaccine has been developed to date. Identification of T-cell target proteins and epitopes is crucial not only for developing a successful immunization strategy, but also for new approaches using adoptive transfer of antigen-specific T-cells. The CMV genome has more than 200 open reading frames potentially coding for as many proteins. Here, we describe a robust, fast, and simple SPOT synthesis strategy, which allowed us to micro-synthesize every possible CD8 T-cell epitope in the entire potential CMV proteome. So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones.
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| http://dx.doi.org/10.1002/bip.20637 | DOI Listing |
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