AI Article Synopsis
- Researchers created a new method called two-injector microchip electrophoresis frontal analysis (MCE-FA) to quickly and accurately study how drugs interact with plasma proteins.
- This method allows for the simultaneous introduction of a reference sample and drug-protein mixture into a microchannel, using separate injectors, which helps to measure interactions without them interfering with each other.
- The study focused on the interaction between heparin and human serum albumin (HSA), finding a binding constant of approximately (1.53 +/- 0.01) x 10(4) M(-1).
Article Abstract
We developed a simple, rapid, and sensitive two-injector microchip electrophoresis frontal analysis (MCE-FA) method for studying drug-plasma protein interactions. In this method, large volumes of a reference sample and drug-plasma protein mixture were simultaneously introduced into the respective sections of the microchannel through the separated injectors and then electrophoresed. Since the reference sample did not meet with the interacting species during migration, it could be used as an external standard. The interaction between heparin and HSA was quantitatively characterized as a model system. The binding constant was found to be (1.53 +/- 0.01) x 10(4) M(-1).
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| http://dx.doi.org/10.1002/elps.200600294 | DOI Listing |
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