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Isolation, identification, and pathogenicity of a goose astrovirus 1 strain from goslings in Jiangsu province, China.
Microb Pathog
January 2025
Jiangsu Agri-Animal Husbandry Vocational College, Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Taizhou 225300, PR China. Electronic address:
Goose astrovirus (GAstV) poses a large threat to the goose industry in China, with two genotypes: goose astrovirus genotype 1 (GAstV-1) and GAstV-2. GAstV-2 causes gout in goslings; however, understanding of GAstV-1 is limited. In this study, the GAstV-1 strain JY202323, was isolated from dead goslings, and its complete genome sequence was obtained using next-generation sequencing.
View Article and Find Full Text PDFCharacterization of the putative yeast mitochondrial triacylglycerol lipase Tgl2.
J Biol Chem
January 2025
Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany. Electronic address:
Mitochondria derive the majority of their lipids from other organelles through contact sites. These lipids, primarily phosphoglycerolipids, are the main components of mitochondrial membranes. In the cell, neutral lipids like triacylglycerides (TAGs) are stored in lipid droplets, playing an important role in maintaining cellular health.
View Article and Find Full Text PDFTopologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolution.
EBioMedicine
January 2025
Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, New Cornerstone Science Foundation, Beijing, 100084, China. Electronic address:
Background: The widespread and evolution of RNA viruses, such as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the importance of fast identification of virus subtypes, particularly in non-laboratory settings. Rapid and inexpensive at-home testing of viral nucleic acids with single-base resolution remains a challenge.
Methods: Topologically constrained DNA ring is engineered as substrates for the trans-cleavage of Cas13a to yield an accelerated post isothermal amplification.
Analysis of pathogen distribution, drug sensitivity and inflammatory indicators related to pelvic infection after hysterectomy.
Eur J Obstet Gynecol Reprod Biol
January 2025
Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, Jiangsu, PR China. Electronic address:
Objective: To analyse the distribution of pathogens, risk factors and inflammatory indicators related to pelvic infection after hysterectomy.
Methods: Patients who underwent hysterectomy at Women's Hospital of Nanjing Medical University between January 2022 and January 2023 were recruited into this study. Vaginal secretions from patients with suspected postoperative pelvic infection were collected for pathogen cultivation, identification, and antimicrobial susceptibility analysis.
Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach.
Viruses
January 2025
Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
The present study aims to better understand the nature of currently circulating GPV strains and their pathological impact on the immune system during natural outbreaks among different duck breeds in Egypt. For this purpose, 99 ducks (25 flocks) of different breeds, aged 14-75 days, were clinically examined, and 75 tissue pools from the thymus, bursa of Fabricius, and spleen were submitted for virus detection and identification. Clinical and postmortem findings were suggestive of GPV infection.
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