Seven isolates of Rickettsia rickettsii were obtained from a skin biopsy, two whole-blood specimens, and from Rhipicephalus sanguineus ticks from eastern Arizona. Molecular typing of seven isolates of R. rickettsii and DNA samples from two other Rh. sanguineus ticks infected with R. rickettsii was conducted by PCR and DNA sequencing of rompA and 12 variable-number tandem repeat regions (VNTRs). All DNA specimens from Arizona were identical to each other and to reference human and Dermacentor andersoni isolates of R. rickettsii from Montana in their rOmpA gene sequences and 10 VNTRs. Two of the twelve VNTRs had differences in the number of repeat sequences in isolates from Arizona compared to those from Montana, thus conferring the novelty of the Rh. sanguineus-associated R. rickettsii.
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http://dx.doi.org/10.1196/annals.1374.114 | DOI Listing |
PLoS One
January 2025
Bio Bureau Biotechnology, Rio de Janeiro, Rio de Janeiro, Brazil.
Monitoring biodiversity on a large scale, such as in hydropower reservoirs, poses scientific challenges. Conventional methods such as passive fishing gear are prone to various biases, while the utilization of environmental DNA (eDNA) metabarcoding has been restricted. Most eDNA studies have primarily focused on replicating results from traditional methods, which themselves have limitations regarding representativeness and bias.
View Article and Find Full Text PDFMol Biol Rep
January 2025
Department of Biomedical Laboratory Science and Management, Vidyasagar University, Midnapore, West Bengal, 721102, India.
Background: Rising antimicrobial resistance (AMR) is an acute public health emergency impeding the clinical efficacy of surgical interventions. Biliary stent placement is one of the routine surgical procedures that rarely lead to infections that are empirically managed by broad-spectrum β-lactams and fluoroquinolones. Critical priority pathogens, such as carbapenem-resistant Escherichia coli challenge treatment outcomes and infection prevention.
View Article and Find Full Text PDFMicrob Drug Resist
January 2025
Department of Medical Microbiology, Ankara University School of Medicine, Ankara, Türkiye.
Colistin- and carbapenem-resistant (ColR CrKp) cause important health problems in pediatric intensive care units (PICUs) due to its ability to harbor multiple resistance genes and spread of high-risk clones. In this study, molecular epidemiological characteristics, transferable resistance genes, and alterations of ColR CrKp isolated from PICU were investigated. Isolates were identified by MALDI-TOF MS, and antimicrobial susceptibility tests were performed using disk diffusion method, gradient strip test, and broth microdilution method.
View Article and Find Full Text PDFJ Fungi (Basel)
January 2025
Kashkin Research Institute of Medical Mycology, North-Western State Medical University Named after I.I. Mechnikov, 191015 Saint Petersburg, Russia.
is an emerging multidrug-resistant fungal pathogen causing nosocomial transmission and invasive infections with high mortality. This study aimed to investigate the genetic relationships, enzymatic activities, and drug-resistance profiles of isolates to evaluate the population and epidemiological diversity of candidiasis in Russia. A total of 112 clinical isolates of were analyzed from May 2017 to March 2023 in 18 hospitals across Saint Petersburg, the Leningrad Region, and Moscow.
View Article and Find Full Text PDFJ Fungi (Basel)
January 2025
Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, Brazil.
We investigated the molecular mechanisms underlying azole resistance in seven isolates that caused candidemia and candiduria in Paraná, Brazil (2016-2022). Biofilm production, antifungal susceptibility testing, multilocus sequence typing, amplification and sequencing of , and quantification of , , and expression levels were performed. Notably, five isolates (71.
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