A fatal case of nosocomial legionellosis in a low prevalence region (Calgary, Alberta, Canada) prompted investigation into the source of infection. Hospital water systems contaminated with Legionella pneumophila have been shown to pose a risk to compromised patients. Typing of an L. pneumophila serogroup 1 strain isolated from the patient using sequence-based typing (SBT) and amplified fragment length polymorphism (AFLP) analysis linked it to a persistent and widespread strain isolated from the hospital water system establishing a nosocomial mode of acquisition. Different SBT and AFLP patterns were determined for non-epidemiologically linked cases and isolates from different hospitals.
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http://dx.doi.org/10.1099/jmm.0.46754-0 | DOI Listing |
Foodborne Pathog Dis
January 2025
Microbiology, Fermentation and Biotechnology Division, ICAR-Central Institute of Fisheries Technology, Cochin, India.
is a recently described species that can be differentiated from . However, in clinical settings, they are frequently misidentified as . In this study, our objective was to conduct genomic characterization and bioinformatics analysis of subsp.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2025
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3 and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus and shared 99.
View Article and Find Full Text PDFmSphere
December 2024
Department of Bioengineering, University of California, San Diego, La Jolla, California, USA.
Unlabelled: Thousands of complete genome sequences for strains of a species that are now available enable the advancement of pangenome analytics to a new level of sophistication. We collected 2,377 publicly available complete genomes of for detailed pangenome analysis. The core genome and accessory genomes consisted of 2,398 and 5,182 genes, respectively.
View Article and Find Full Text PDFEur J Clin Microbiol Infect Dis
December 2024
Department of Microbiology and Infection Control, National Reference Centre for Legionella Pneumophila, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Laarbeeklaan 101, 1090, Brussels, Belgium.
Whole genome sequencing (WGS) marks a turning point for outbreak investigations for microorganisms related to public health matters, like Legionella pneumophila (Lp). Here, we evaluated the available Lp WGS typing tools for isolates of previously documented Belgian outbreaks, as well as small groups of related and non-related isolates. One reference strain and 77 clinical and environmental isolates were evaluated.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
December 2024
Department of Fermentation Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.
An obligately anaerobic, Gram-stain-negative, non-spore-forming and non-motile rod (strain LPYR103-Pre) was isolated from a two-phase methane fermentation system. Using 16S rRNA gene sequence-based phylogenetic analysis, strain LPYR103-Pre was classified in the genus . The 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization between strain LPYR103-Pre and its phylogenetically nearest species JCM 30867 - were 94.
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