Differentiation of mesenchymal stem cells (MSCs) into anterior cruciate ligament (ACL) cells is regulated by many factors. Mechanical stress affects the healing and remodeling process of ACL after surgery in important ways. Besides, co-culture system had also showed the promise to induce MSCs toward different kinds of cells on current research. The purpose of this study was to investigate the gene expression of ACL cells' major extracellular matrix (ECM) component molecules of MSCs under three induction groups. In addition, to follow our previous study, cell electrophoresis technique and mRNA level gene expression of MSC protein were also used to analyze the differentiation of MSCs. The results reveal that specific regulatory signals which released from ACL cells appear to be responsible for supporting the selective differentiation toward ligament cells in co-culture system and mechanical stress promotes the secretion of key ligament ECM components. Therefore, the combined regulation could assist the development of healing and remolding of ACL tissue engineering. Furthermore, this study also verifies that cell electrophoresis could be used in investigation of cell differentiation. Importantly, analysis of the data suggests the feasibility of utilizing MSCs in clinical applications for repairing or regenerating ACL tissue.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2006.10.170 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microgap Formation in Conical Implant-Abutment Connections Under Oblique Loading: Influence of Cone Angle Mismatch Through Finite Element Analysis.
Clin Implant Dent Relat Res
February 2025
Minnesota Dental Research Center for Biomaterials and Biomechanics, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
Objectives: This study evaluated different designs of the conical implant-abutment connection (IAC) and their resistance to microgap formation under oblique loads as specified by the ISO standard for testing dental implants. Also evaluated was the effect of deviations from the ISO specifications on the outcomes.
Methods: Finite element analysis was conducted to compare the microgap formation and stress distribution among three conical IAC designs (A, B, and C) in two loading configurations: one compliant with ISO 14801 and one with a modified load adaptor (non-ISO).
Flow environment affects nutrient transport in soft plant roots.
Soft Matter
January 2025
Microfluidics and Microscale Transport Processes Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India.
This work estimates Michaelis-Menten kinetics parameters for nutrient transport under varying flow rates in the soft roots of Indian mustard () using a plant fluidic device. To find the metallic components within the roots, inductively coupled plasma mass spectrometry (ICP-MS) analysis was performed. The flow rate-dependent metabolic changes were examined using Raman spectral analysis.
View Article and Find Full Text PDFBiomechanical analysis of three kinds of rigid internal fixation methods for condylar head fractures.
Hua Xi Kou Qiang Yi Xue Za Zhi
February 2025
Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Bengbu Medical University, Bengbu 233004, China.
Objectives: This study aims to analyze the biomechanics of three kinds of rigid internal fixation methods for condylar head fractures.
Methods: A three dimensional finite element model of the normal mandible was constructed. It was then used to prepare condylar head fracture finite element model and three kinds of rigid internal fixation finite element model (unilateral tension screw, bilateral tension screw, tension screw+titanium plate).
Efficiency of Polyethylene Terephthalate Glycol Thermoplastic Material to Functional and Expansion Forces in Orthodontic Applications: An Experimental Study.
ScientificWorldJournal
January 2025
Department of Orthodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq.
While polyethylene terephthalate glycol (PETG) is widely used in orthodontic appliances such as clear aligners and retainers, there is limited experimental data assessing its performance under functional stresses, such as those encountered during dental movements and palatal expansion. This study aims to evaluate the ability of PETG thermoplastic material to withstand deformation under functional and expansion forces, specifically within the context of orthodontic applications. To estimate the firmness of the screw within the appliance, a universal Instron testing machine was used to record the forces released by each activation of the expander within the upper part of 10 clear modified twin blocks (MTBs) made from PETG and compare it with that released by 10 conventional twin blocks (CTBs).
View Article and Find Full Text PDFDiscovering non-associated pressure-sensitive plasticity models with EUCLID.
Adv Model Simul Eng Sci
January 2025
Department of Mechanical and Process Engineering, Institute for Mechanical Systems, ETH Zürich, Zürich, 8092 Switzerland.
We extend (EUCLID Efficient Unsupervised Constitutive Law Identification and Discovery)-a data-driven framework for automated material model discovery-to pressure-sensitive plasticity models, encompassing arbitrarily shaped yield surfaces with convexity constraints and non-associated flow rules. The method only requires full-field displacement and boundary force data from one single experiment and delivers constitutive laws as interpretable mathematical expressions. We construct a material model library for pressure-sensitive plasticity models with non-associated flow rules in four steps: (1) a Fourier series describes an arbitrary yield surface shape in the deviatoric stress plane; (2) a pressure-sensitive term in the yield function defines the shape of the shear failure surface and determines plastic deformation under tension; (3) a compression cap term determines plastic deformation under compression; (4) a non-associated flow rule may be adopted to avoid the excessive dilatancy induced by plastic deformations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!