AI Article Synopsis
- The study focuses on the expression levels of adrenomedullin (ADM) and its receptor components in pheochromocytoma using RT-PCR.
- The results showed that both ADM and its receptor mRNA are significantly more prevalent in pheochromocytoma tissues compared to normal adrenal medulla.
- Additionally, ADM appears to inhibit the growth of pheochromocytoma cells, indicating a potential local (autocrine or paracrine) role in the adrenal environment.
Article Abstract
This study investigates the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in pheochromocytoma by reverse transcriptase polymerase chain reaction (RT-PCR) and its effect on the proliferation of pheochromocytoma cells by MTT. The mRNA expression of ADM and its receptor RAMP2/CRLR was present in normal adrenal medulla and pheochromocytoma tissues. The mRNA expression of ADM, RAMP2, and CRLR is markedly higher in pheochromocytomas than in normal medulla. ADM inhibits the proliferation of human pheochromocytoma cells and exerts a possible autocrine or paracrine effect in the adrenal.
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| http://dx.doi.org/10.1196/annals.1353.030 | DOI Listing |
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Article Synopsis
- The study focuses on the expression levels of adrenomedullin (ADM) and its receptor components in pheochromocytoma using RT-PCR.
- The results showed that both ADM and its receptor mRNA are significantly more prevalent in pheochromocytoma tissues compared to normal adrenal medulla.
- Additionally, ADM appears to inhibit the growth of pheochromocytoma cells, indicating a potential local (autocrine or paracrine) role in the adrenal environment.
[Expression of adrenomedullin and its receptor mRNA in the tissues of normal adrenal medulla and pheochromocytoma].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
August 2005
Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Objective: To investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.
Methods: Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction.
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