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| http://dx.doi.org/10.1073/pnas.0608935103 | DOI Listing |
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Interaction proteomics analysis to provide insight into TFAMoplex-mediated transfection.
J Control Release
September 2024
ETH Zurich, Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Vladimir-Prelog-Weg 3, 8093 Zurich, Switzerland.. Electronic address:
In an earlier investigation, our group introduced the TFAMoplex, a transfection agent based on the mitochondrial transcription factor A (TFAM) protein, which complexes DNA into nanoparticles. The original TFAMoplex further contained a bacterial phospholipase to achieve endosomal escape, and the vaccinia-related kinase 1 (VRK1), which significantly boosted the transfection efficiency of the system by an unknown mechanism. This study aims at replacing VRK1 within the TFAMoplex with dynein light chain proteins, specifically RP3, to directly tether the complexes to the dynein motor complex for enhanced cytosolic transport.
View Article and Find Full Text PDFThe physical and cellular mechanism of structural color change in zebrafish.
Proc Natl Acad Sci U S A
June 2024
HHMI, Janelia Research Campus, Ashburn, VA 20147.
Article Synopsis
- Animals use intracellular guanine crystals to create vibrant colors for communication, camouflage, and temperature regulation through light interference.
- Research focused on zebrafish showed that norepinephrine-induced color changes involve a coordinated 20° tilt of these crystal arrays, affecting their packing and how light interacts with them.
- The study uncovered that microtubules and the protein dynein play critical roles in modifying crystal angles, while intracellular cAMP also regulates the color change dynamics.
The association and underlying mechanism of the digit ratio (2D:4D) in hypospadias.
Asian J Androl
July 2024
Department of Urology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200062, China.
The second-to-fourth digit (2D:4D) ratio is thought to be associated with prenatal androgen exposure. However, the relationship between the 2D:4D ratio and hypospadias is poorly understood, and its molecular mechanism is not clear. In this study, by analyzing the hand digit length of 142 boys with hypospadias (23 distal, 68 middle, and 51 proximal) and 196 controls enrolled in Shanghai Children's Hospital (Shanghai, China) from December 2020 to December 2021, we found that the 2D:4D ratio was significantly increased in boys with hypospadias ( P < 0.
View Article and Find Full Text PDFCondensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization.
EMBO J
October 2023
Department of Chemistry, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, Paris, France.
The localization of RNAs in cells is critical for many cellular processes. Whereas motor-driven transport of ribonucleoprotein (RNP) condensates plays a prominent role in RNA localization in cells, their study remains limited by the scarcity of available tools allowing to manipulate condensates in a spatial manner. To fill this gap, we reconstitute in cellula a minimal RNP transport system based on bioengineered condensates, which were functionalized with kinesins and dynein-like motors, allowing for their positioning at either the cell periphery or centrosomes.
View Article and Find Full Text PDFSolution NMR Backbone Assignment of the C-Terminal Region of Human Dynein Light Intermediate Chain 2 (LIC2-C) Unveils Structural Resemblance with Its Homologue LIC1-C.
Magnetochemistry
July 2023
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Dynein, a homodimeric protein complex, plays a pivotal role in retrograde transportation along microtubules within cells. It consists of various subunits, among which the light intermediate chain (LIC) performs diverse functions, including cargo adaptor binding. In contrast to the vertebrate LIC homolog LIC1, LIC2 has received relatively limited characterization thus far, despite partially orthogonal functional roles.
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