Capillary electrophoresis was successfully applied for separation of the enantiomers of N-benzyloxycarbonyl-alpha-aminophosphonic and alpha-aminophosphinic acids as well as their ethyl and phenyl monoesters with the use of a range of commercially available cyclodextrins (alpha, beta and hydroxypropyl-gamma-cyclodextrins) as chiral selectors. The dependence of effectiveness of separation on type and concentration of these chiral selectors as well as on pH of background electrolyte was examined in some detail.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2006.10.065DOI Listing

Publication Analysis

Top Keywords

capillary electrophoresis
8
chiral selectors
8
enantiodifferentiation n-benzyloxycarbonylaminophosphonic
4
n-benzyloxycarbonylaminophosphonic phosphinic
4
phosphinic acids
4
acids esters
4
esters cyclodextrins
4
cyclodextrins capillary
4
electrophoresis capillary
4
electrophoresis applied
4

Similar Publications

In most cases, the diagnosis of diabetes in animal models is based solely on blood glucose levels. While hemoglobin A1c (HbA1c) is widely used in the diagnosis of diabetes in humans, it is rarely measured in mice in diabetes research. This is thought to be because there are no established reference values for mouse HbA1c, as well as the fact that there are very few reports on the variability and reproducibility of measurements taken using different devices.

View Article and Find Full Text PDF

From dual nucleic acid co-extraction to co-sequencing: A highly integrated next-generation forensic DNA and RNA sequencing experimental workflow.

Forensic Sci Int Genet

January 2025

Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; School of Forensic Medicine, Kunming Medical University, Kunming 650500, China. Electronic address:

DNA and RNA markers are significant in forensic practices, such as individual and body fluid identification. However, forensic DNA and RNA markers were separately analyzed in most forensic experiments, which resulted in large amounts of sample consumption, complex procedures, and weak inter-evidence correlation. While several integrated methods based on capillary electrophoresis and next-generation sequencing technologies were reported, integrated procedures were mostly on nucleic acid co-extraction, co-electrophoresis, or co-sequencing, and the number and type of markers co-tested were limited.

View Article and Find Full Text PDF

Background: Hemoglobin (Hb) Hekinan is a prevalent α-globin variant frequently missed in thalassemia screening centers using high-performance liquid chromatography (HPLC) or capillary electrophoresis. This study aims to investigate the hematological and molecular characteristics of Hb Hekinan in a large cohort.

Methods: Hb variants were identified using isoelectric focusing (IEF) and HPLC.

View Article and Find Full Text PDF

Objectives: This study reported a large cohort of fetal blood analysis of various hemoglobinopathies.

Methods: A total of 371 fetal blood specimens were recruited. Complete blood count and hemoglobin (Hb) analysis using capillary electrophoresis were performed.

View Article and Find Full Text PDF

Background And Objective: Serum protein electrophoresis (SPEP) plays a critical role in diagnosing diseases associated with M-proteins. However, its clinical application is limited by a heavy reliance on experienced experts.

Methods: A dataset comprising 85,026 SPEP outcomes was utilized to develop artificial intelligence diagnostic models for the classification and localization of M-proteins.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!