AI Article Synopsis
- Gamma-irradiation leads to cell death (apoptosis) and stops cell division (cell cycle arrest) in eukaryotic cells, specifically affecting the G2 phase.
- Olomoucine, a new drug that inhibits certain proteins (cyclin-dependent kinases), was studied in the Raji cell line for its effects on cells treated with gamma-irradiation.
- The combination of olomoucine and gamma-irradiation intensified cell cycle arrest and reduced survival rates, while also delaying the apoptotic process but hindering cell growth and DNA synthesis in remaining cells.
Article Abstract
Gamma-irradiation leads to apoptosis and cell cycle arrest in eukaryotic cells. Olomoucine is a novel purine analog acting as a cyclin-dependent kinase inhibitor. The effects of olomoucine in gamma-irradiation mediated cell growth inhibition and apoptosis were studied in the Raji cell line (Burkitt's lymphoma). Gamma-irradiation caused a G2 arrest, increasing the G2/M fragment of the cells. Apoptosis by gamma-irradiation was apparent both by DNA-electrophoresis and PARP-1 cleavage. The combination of olomoucine with irradiation caused an increased G2 arrest and decreased cell survival and DNA synthesis in the non-apoptotic fraction of the remaining cells. Irradiation, as well as olomoucine and the combination of both, induced apoptosis. It seems that olomoucine delays the apoptotic process and inhibits DNA fragmentation, but it decreased survival, cell cycle progression and proliferation of irradiated cells.
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