AI Article Synopsis
- TFII-I is a key transcription factor that influences gene modulation by interacting with various nuclear factors and chromatin remodeling complexes.
- Recent research shows that TFII-I plays significant roles in cell functions and the development of mouse embryos.
- Studies involving the overexpression of TFII-I in mouse embryonic fibroblasts identified several genes related to immune response, signaling pathways, and transcription regulation, confirming TFII-I's involvement in multiple cellular processes.
Article Abstract
TFII-I is a founding member of a family of helix-loop-helix transcription factors involved in modulation of genes through interaction with various nuclear factors and chromatin remodeling complexes. Recent studies indicate that TFII-I performs important function in cell physiology and mouse embryogenesis. In order to understand its molecular role, TFII-I was overexpressed in primary mouse embryonic fibroblasts (MEFs) and alterations in gene expression were monitored with a mouse 16 K oligonucleotide microarray. These studies allowed us to identify genes that lie downstream of TFII-I-dependent pathways. Among the modulated candidates were genes involved in the immunity response, catalytic activity, signaling pathways and transcriptional regulation. Expression of several candidates including those for the interferon-stimulated protein (G1p2), small inducible cytokine A7 (Ccl7), ubiquitin-conjugating enzyme 8 (Ube2l6), cysteine-rich protein (Csrp2) and Drosophila delta-like 1 homolog (Dlk1) were confirmed by real-time PCR. The obtained results suggest that TFII-I participates in multiple signaling and regulatory pathways in MEFs.
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| http://dx.doi.org/10.1002/jez.b.21134 | DOI Listing |
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