Aim: This study tested the null hypothesis that there is no difference on microleakage of dentin-bordered composite restorations using single or double coats of adhesive from one-bottle adhesive systems.

Methods And Materials: The enamel surface was removed from freshly extracted bovine teeth, and standardized Class V cavities (3 x 3 x 1.5 mm) were made at the cervical areas of buccal surfaces. Teeth were restored and grouped according to type of adhesive systems [Prime Bond 2.1 (PB2.1), Prime & Bond NT (PBNT), and Single Bond (SB)] and to the number of coats (one or two) to be used. The restorations were polished and immersed in a 0.5% aqueous solution of basic fuchsin for four hours. Teeth were then sectioned and the most infiltrated section of each tooth was selected under magnification, scanned, and quantitatively analyzed using a computer program. Data were analyzed using two-way analysis of variance (ANOVA) and Student-Newman-Keuls (a=0.05).

Results: Groups without filler content (PB2.1 and SB) showed no difference in microleakage using single or double coats. However, PBNT (with nanofiller) showed statistically less microleakage when only one coat was applied. The influence of the number of coats of the adhesive systems on dentin margin microleakage was material dependent.

Conclusion: All adhesive systems demonstrated microleakage, however, it could be minimized using two coats of non-filled or one coat of a filled adhesive system.

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