Objective: To develop a method to rapidly detect influenza virus type A by fluorescence real-time quantitative RT-PCR.
Methods: According to conservative sequence of influenza virus type A M2 gene, a pair primers and Taqman probe were designed, respectively. After the quantitative curve of the assay were established using tenfold serial dilution of TCID50, the sensitivity and the specificity of clinic samples were determined.
Results: The detection sensitivity of the assay was 2.56 x 10(-5) TCID50 and the regression coefficient of the quantitative curve was 0.999. The specificity was determined by testing five other specimens, all of which yielded negative results.
Conclusion: The detection system based on real-time RT-PCR was rapid, sensitive and steady, which could be used to detect the clinic samples.
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