Objective: To study the effects of PM10 and the concentrated culture suspension of RAW264.7 cells treated with PM10 on the secretion of tumor necrosis factor-alpha (TNF-alpha), interleukin-6(IL-6) and interleukin-8(IL-8) in the human lung fibroblasts( HLF).
Methods: PM10 were collected during heating period in the urban area of Beijing. HLF and mouse alveolar macrophage cell (RAW264.7) were used. The two ways of exposed cells to PM10 were used: (1) HLF were treated with the different concentration of PM10 for 24h. (2) HLF were stimulated by the added the suspension of RAW264.7 cell exposed to different concentrations of PM10. Cytotoxicity of PM10 was measured by MTT assay. The levels of inflammatory cytokines TNF-alpha, IL-6 and IL-8 were determined by the radioimmunity assay.
Results: After treated for 24h, PM10 showed the cytotoxicity in HLF and RAW264.7 cells, which was characterized by increase of the viability of cells at low concentration of PM10, and decrease at high doses. PM10 induced the secretion of TNF-alpha, IL-6 and IL-8 in HLF in a dose-dependent manner. The concentrated culture medium of RAW264.7 cells treated with PM10 also stimulated the secretion of TNF-alpha, IL-6 and IL-8 in HLF.
Conclusion: PM10 is cytotoxic to HLF and RAW264.7 cells. PM10 induced the secretion of TNF-alpha, IL-6 and IL-8 in HLF. This effect was also observed by treatment of HLF with the concentrated culture medium of RAW264.7 cell exposed to PM10.
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