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Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging.
Biophys Rep
December 2024
Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Membrane proteins often need to be inserted into or attached to the cell membrane to perform their functions. Understanding their transmembrane topology and conformational dynamics during insertion is crucial for elucidating their roles. However, it remains challenging to monitor nanoscale changes in the insertion depth of individual proteins in membranes.
View Article and Find Full Text PDFSingle-Molecule Tracking and Super-Resolution Microscopy Unveil Actin-Driven Membrane Nanotopography Shaping Stable Integrin Adhesions in Developing Tissue.
Cytoskeleton (Hoboken)
January 2025
Interdisciplinary Institute for Neuroscience, Université Bordeaux, CNRS, Bordeaux, France.
Single molecule tracking and super-resolution microscopy of integrin adhesion proteins and actin in developing Drosophila muscle attachment sites reveals that nanotopography triggered by Arp2/3-dependent actin protrusions promotes stable adhesion formation. The nanodomains formed during this process confine the diffusion of integrins and promote their immobilization. Spatial confinement is also applied to the motion of actin filaments, resulting in enhanced mechanical connection with the integrin adhesion complex.
View Article and Find Full Text PDFDynamics of Nucleosomes and Chromatin Fibers Revealed by Single-Molecule Measurements.
BMB Rep
January 2025
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan (44919), Republic of Korea.
The nucleosome is the fundamental structural unit of chromosome fibers. A DNA wraps around a histone octamer to form a nucleosome, while neighboring nucleosomes interact to form higher-order structures and fit gigabase-long DNAs into a small volume of the nucleus. Nucleosomes interrupt the access of transcription factors to a genomic region, and provide regulatory controls of gene expression.
View Article and Find Full Text PDFTowards effective functionalization of nanopores/nanochannels: the role of amidation reactions.
Chem Commun (Camb)
January 2025
State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
In recent years, researchers have drawn inspiration from natural ion channels to develop various artificial nanopores/nanochannels, including solid-state and biological. Through imitating the precise selectivity and single molecule sensing exhibited by natural ion channels, nanopores/nanochannels have been widely used in many fields, such as analyte detection, gene sequencing and so on. In these applications, the surface functionalization of nanopores/nanochannels directly determines the effectiveness in quantitative analysis and single molecule detection.
View Article and Find Full Text PDFSingle-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF.
Nat Commun
January 2025
State Key Laboratory of Membrane Biology, Beijing Frontier Research Center of Biological Structure, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, China.
SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptor) proteins are the minimal machinery required for vesicle fusion in eukaryotes. Formation of a highly stable four-helix bundle consisting of SNARE motif of these proteins, drives vesicle/membrane fusion involved in several physiological processes such as neurotransmission. Recycling/disassembly of the protein machinery involved in membrane fusion is essential and is facilitated by an AAA+ ATPase, N-ethylmaleimide sensitive factor (NSF) in the presence of an adapter protein, α-SNAP.
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