To clarify the molecular basis of toxicities of industrial chemicals, it is demanded to develop appropriate methods whereby their cellular target molecules can be directly identified. In the present study, we focused on target proteins of heavy metals and established the method to detect them using a combination of metal-chelating column chromatography and a subsequent analysis by electrophoresis. Protein samples prepared from HeLa cells were applied to the Zn- or Cd-chelating column, and the bound proteins were analyzed by SDS-polyacrylamide gel electrophoresis followed by either silver staining, or fluorography when using radiolabel protein samples. Among several protein species trapped in the columns, a 36-kDa protein apparently has an affinity to both Zn and Cd, indicating the possibility that Cd can exchange essential Zn on this protein. These results suggest that the established method is useful for the target protein screening and further analyses of separated proteins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2486/indhealth.44.674 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Uncovering mercury accumulation and the potential for bacterial bioremediation in response to contamination in the Singalila National Park.
Sci Rep
January 2025
Molecular Biology and Tissue Culture Laboratory, Department of Tea Science, University of North Bengal, Siliguri, West Bengal, India.
Several recent investigations into montane regions have reported on excess mercury accumulation in high-altitude forest ecosystems. This study explored the Singalila National Park, located on the Singalila ridge of the Eastern Himalayas, revealing substantial mercury contamination. Particular focus was on Sandakphu (3636 m), the highest peak in West Bengal, India.
View Article and Find Full Text PDFUltrasensitive and selective impedance paper-based analytical device through Dual-C imprinted sensor for determination of carcinoembryonic antigen and C-reactive protein.
Mikrochim Acta
January 2025
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand.
Carcinoembryonic antigen (CEA) and C-reactive protein (CRP) are biomacromolecules known as cancer and inflammatory markers. Thus, they play a crucial role in early cancer diagnosis, post-treatment recurrence detection, and tumor risk assessment. This paper describes the development of an ultrasensitive and selective imprinted paper-based analytical device (PAD) as impedance sensor for determination of CEA and CRP in serum samples for point-of-care testing (POCT).
View Article and Find Full Text PDFFish active packaging with ZnO/Fe-MMT nanoparticles.
Sci Rep
January 2025
Nanotechnology Department, Faculty of Science, Urmia University, Urmia, Iran.
Today, active packaging has become essential to increase food safety and decrease food spoilage. In this study, the aim was to delay spoilage and increase the shelf life of rainbow fish fillets with a new hybrid nanocomposite active packaging. Packaging was fabricated with Ethylene vinyl acetate and active compounds such as rosemary extract, zinc oxide nanoparticles, and modified iron (Fe-MMT).
View Article and Find Full Text PDFEcofriendly remediation of cadmium, lead, and zinc using dead cells of Microcystis aeruginosa.
Sci Rep
January 2025
Hydrobiology Lab, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.
The utilization of cyanobacteria toxin-producing blooms for metal ions adsorption has garnered significant attention over the last decade. This study investigates the efficacy of dead cells from Microcystis aeruginosa blooms, collected from agricultural drainage water reservoir, in removing of cadmium, lead, and zinc ions from aqueous solutions, and simultaneously addressing the mitigation of toxin-producing M. aeruginosa bloom.
View Article and Find Full Text PDFSalicylic acid confers cadmium tolerance in wheat by regulating photosynthesis, yield and ionic homeostasis.
Sci Rep
January 2025
Institute of Field and Vegetable Crops, National Institute of the Republic of Serbia, Maxim Gorki, 30, Novi Sad, 21000, Serbia.
Wheat (Triticum aestivum L.) productivity and quality can be threatened by soil cadmium (Cd) contamination, posing a concern to food security. Salicylic acid (SA) is an endogenously produced signaling molecule that activates the defense system imparting abiotic stress tolerance in plants.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!