We assessed the impact of the use of nasal swabs or nasopharyngeal aspirates and the time from specimen collection to storage at -70 degrees C on bacterial isolation. Haemophilus influenzae was isolated significantly less often from swabs than from nasopharyngeal aspirates. Samples in transit for >3 days were half as likely to grow Streptococcus pneumoniae and H. influenzae as those in transit for < or =3 days. There was no statistically significant difference for either Moraxella catarrhalis or Staphylococcus aureus.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828972 | PMC |
http://dx.doi.org/10.1128/JCM.01131-06 | DOI Listing |
Viruses
December 2024
Smorodintsev Research Institute of Influenza, 197376 Saint Petersburg, Russia.
Human seasonal coronaviruses (hCoVs) are a group of viruses that affect the upper respiratory tract. While seasonal patterns and the annual variability of predominant hCoV species are well-documented, their genetic and species diversity in St. Petersburg and across Russia remains largely unexplored.
View Article and Find Full Text PDFPathogens
January 2025
Center for Advanced Molecular Detection, 59th Medical Wing/Science & Technology, Joint Base San Antonio, Lackland, TX 78236, USA.
Background: Respiratory viral infections are a major public health challenge and the most diagnosed medical condition, particularly for individuals living in close proximity, like military personnel. We compared the sensitivity and specificity of the Biomeme Franklin and Truelab RT-PCR thermocyclers to determine which platform is more sensitive and specific at detecting SARS-CoV-2 and influenza A and B viruses.
Methodology: RNA extracted from nasopharyngeal swabs of infected and uninfected individuals was tested on the Biomeme Franklin at Lackland and the Truelab at Wright Patterson Air Force bases.
Microorganisms
January 2025
Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 48, 50134 Florence, Italy.
The mechanisms underlying post-acute sequelae of SARS-CoV-2 infection (PASC) are a topic of debate. This study examined the presence of SARS-CoV-2 microRNA (miRNA)-like small RNAs in extracellular fluids and their potential link to PASC by using a quantitative stem-loop RT-PCR MiRNA assay. Initially, it was demonstrated that three previously identified SARS-CoV-2 miRNA-like small RNAs, specifically svRNA 1 and 2 and miR-07a, were significantly expressed in infected cells in vitro and released into the supernatant following infection by different SARS-CoV-2 variants.
View Article and Find Full Text PDFSci Rep
January 2025
Research and Innovation Unit, Costa del Sol University Hospital, Autovía A-7, km 187, Marbella, 29603, Spain.
The resurgence of COVID-19 and the rise in severe outcomes emphasize the need for reliable prognostic markers to guide patient care and optimize ICU and hospital resources. This study investigates the potential of nasopharyngeal swabs to identify biomarkers that predict ICU admission or death in hospitalized COVID-19 patients. We analyzed nasopharyngeal exudates from 95 hospitalized patients in 2020 using high-plex RNA quantification on the NanoString nCounter platform.
View Article and Find Full Text PDFBiomarkers
January 2025
Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Division of Emergency Medicine, Campus Virchow-Klinikum and Campus Charité Mitte, Berlin, Germany.
Background: Testing for (SA) colonization in emergency department (ED) patients may guide prevention strategies against hospital acquired infections (HAI). This study determined the prevalence of SA carriers in a general ED population, characterized the population, and identified predictors for SA colonization.
Methods: A prospective monocentric observational cohort study in a tertiary care hospital collected nasopharyngeal swabs in 1,000 adult patients.
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