AI Article Synopsis
- The study aimed to evaluate the quality of reborn barks and the antihypertensive effects of pinoresnol diglucoside (PDG) across three rebirth cycles.
- Using specific methods, the analysis showed that PDG levels initially increased after the first rebirth but decreased significantly by the third.
- The researchers recommend limiting the girdling process to two cycles with a minimum five-year interval between them for better quality and PDG enhancement.
Article Abstract
Objective: To evaluate the reborn barks quality, antihypertensive effective component of pinoresnol diglucoside (PDG) in three times reborn barks were determined.
Methods: A YWG C18 column (10 microm, 250 x 4.6 mm) was used with a mobile phase of methanol-water (30:75) and flow rate of 1.0 (ml/min). The detective wave-length was set at 277nm and the column temperature at room temperature.
Results: PDG in the first reborn barks are slightly higher than the original ones, and in the second reborn barks are similar with the barks before girdling (the fist reborn barks), but in the third reborn barks, PDG are much lower than the barks before girdling (the second reborn barks).
Conclusion: In order to ensure reborn barks quality, we suggest that the girdling bark regeneration can be made two times, the time between the first and the second girdling is not less than five years. PDG in the third reborn barks should be enhanced.
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