Background: We have developed a functional genomics approach based on expression cloning in Xenopus oocytes to identify plant transporter function. We utilized the full-length cDNA databases to generate a normalized library consisting of 239 full-length Arabidopsis thaliana transporter cDNAs. The genes were arranged into a 96-well format and optimized for expression in Xenopus oocytes by cloning each coding sequence into a Xenopus expression vector.
Results: Injection of 96 in vitro transcribed cRNAs from the library in pools of columns and rows into oocytes and subsequent screening for glucose uptake activity identified three glucose transporters. One of these, AtSTP13, had not previously been experimentally characterized.
Conclusion: Expression of the library in Xenopus oocytes, combined with uptake assays, has great potential in assignment of plant transporter function and for identifying membrane transporters for the many plant metabolites where a transporter has not yet been identified.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1637106 | PMC |
| http://dx.doi.org/10.1186/1746-4811-2-17 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The stoichiometry of the α4β2 neuronal nicotinic acetylcholine receptors determines the pharmacological properties of the neonicotinoids, and recently introduced butenolide and sulfoximine.
Neurotoxicology
January 2025
Laboratoire Physiologie, Ecologie and Environnement (P2E), Université d'Orléans, UR 1207, USC-INRAE 1328, 1 rue de Chartres, 45067 Orléans, France; Institut Universitaire de France (IUF), 1 rue Descartes 75005 Paris, France. Electronic address:
Although neonicotinoids were considered safe for mammals for many decades, recent research has proven that these insecticides can alter cholinergic functions by interacting with neuronal nicotinic acetylcholine (ACh) receptors (nAChRs). One such receptor is the heteromeric α4β2 nAChR, which exists under two different stoichiometries: high sensitivity and low sensitivity α4β2 nAChRs. To replace these insecticides, new classes of insecticides have been developed, such as, sulfoximine, sulfoxaflor, and the butanolide, flupyradifurone.
View Article and Find Full Text PDFHuman α10 nicotinic acetylcholine receptor subunits assemble to form functional receptors.
J Biol Chem
January 2025
School of Biological Sciences, University of Utah, Salt Lake City, Utah, USA; Department of Psychiatry, University of Utah, Salt Lake City, Utah, USA; George E. Whalen Veterans Affairs Medical Center, Salt Lake City, Utah, USA.
Nicotinic acetylcholine receptors (nAChRs) are pentameric ligand-gated ion channels. In mammals, there are 16 individual nAChR subunits allowing for numerous possible heteromeric compositions. nAChRs assembled from α7 or α9 subunits will form as homopentamers.
View Article and Find Full Text PDFRole of mutation G255A in modulating pyrethroid sensitivity in insect sodium channels.
Int J Biol Macromol
January 2025
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China; School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Danzhou 571700, China. Electronic address:
A voltage-gated sodium channel (VGSC) plays a crucial role in insect electrical signals, and it is a target for various naturally occurring and synthesized neurotoxins, including pyrethroids and dichlorodiphenyltrichloroethane. The type of agent is typically widely used to prevent and control sanitary and agricultural pests. The perennial use of insecticides has caused mutations in VGSCs that have given rise to resistance in most insects.
View Article and Find Full Text PDFRuvbl1 silencing affects reproduction of the corn planthopper, Peregrinus maidis.
PLoS One
January 2025
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, United States of America.
Ruvbl1 (also known as TIP49, Pontin) encodes an ATPase of the AAA+ protein superfamily involved in several cellular functions, including chromatin remodeling, control of transcription, and cellular development (motility, growth, and proliferation). While its role has been well established in model organisms including vertebrates and invertebrates (e.g.
View Article and Find Full Text PDFGlabridin Hypnosis in Zebrafish Larvae Is Associated With Effects on Multiple Anesthetic Target Receptors.
Anesth Analg
November 2024
From the Department of Anesthesia Critical Care & Pain Medicine, Massachusetts General Hospital, Boston, Massachusetts.
Background: R-Glabridin is a major flavonoid of licorice (Glycyrrhiza glabra) root and known to modulate GABAA receptors, which are targets of many clinical hypnotics. However, R-glabridin hypnotic activity has not been reported in animals.
Methods: Inverted photomotor responses (IPMRs) were used to assess the hypnotic effects of natural R-glabridin and synthetic R/S-glabridin in wild-type zebrafish larvae and transgenic larvae lacking functional GABAA receptor β3 subunits (β30/0).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!