AI Article Synopsis
- The study aimed to investigate how chronic, unpredictable mild stress affects the expression of specific proteins (PKA and P-CREB) in the hippocampus of rats, and whether the antidepressant fluoxetine can counteract these effects.
- Thirty-six male rats were divided into three groups: a normal control group, a model group exposed to stress, and a fluoxetine group, with varying conditions and assessments using immunohistochemistry and Western blot techniques.
- Results showed that stressed rats had lower levels of PKA and P-CREB proteins compared to controls, but those treated with fluoxetine exhibited higher protein levels than the stressed group, indicating fluoxetine's effectiveness in reversing stress-induced changes.
Article Abstract
Objective: To observe the effect of chronic unpredicted sequence of mild stress on the expression of cAMP-dependent protein kinase A(PKA) and phosphorylated cAMP-responsive element binding protein (P-CREB) in hippocampus of rats and the antagonism of antidepressors (fluoxetine).
Methods: Thirty-six male Sprague Dawley rats were randomly and equally allocated to 3 groups: A normal control group, a model group, and a fluoxetine group. All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressors. The different distribution and expression of PKA and P-CREB in the hippocampus of rats in different groups were investigated with immunohistochemistry and Westernblot technique.
Results: The positive PKA and P-CREB cells in the hippocampus of normal controls were the pyramidal cells and the granule cells. The PKA and P-CREB protein expression levels in the hippocampus of model rats were significantly lower than those of the normal controls (P<0.05). The PKA and P-CREB protein expression levels in the hippocampus of the fluoxetine group were significantly higher than those of the model group (P<0.05).
Conclusion: Chronic unpredicted mild stress can affect the PKA and P-CREB expression in hippocampus of rats and fluoxetine has antagonism against it.
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