Plant Physiol Biochem
C.N.R. Istituto Chimica del Riconoscimento Molecolare c/o Istituto di Biochimica e Biochimica Clinica, Università Cattolica S. Cuore, L.go F. Vito, 1, 00168 Rome, Italy.
Published: October 2006
A 23-kDa antifungal thaumatin-like protein was isolated and purified from Cassia didymobotrya (Fres.) cell cultures for the first time. The protein was secreted in the culture medium, but it could be also isolated after elution of whole cells with a 0.5 M CaCl(2) solution. Treatment of the cells with laminarin oligosaccharides or salicylic acid, but not with NaCl, resulted in enhancement of expression of the protein. A rapid purification protocol was used based on cationic exchange chromatography. The protein, with a highly basic character (pI 10), has an exact molecular mass of 23034 Da, as determined by MALDI-ToF mass spectrometry analysis. N-terminal sequencing of the intact polypeptide and the sequencing of two internal tryptic peptides indicated significant identity with other thaumatin-like proteins (TLP). The protein exerted antifungal activity towards some Candida species showing EC(50) values comparable to those of other antifungal TLPs. The collected data lead to classify this TLP as a new PR-5 protein.
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http://dx.doi.org/10.1016/j.plaphy.2006.09.008 | DOI Listing |
New Phytol
December 2024
College of Plant Protection, Hebei Agricultural University/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas, 071000, Baoding, China.
Thaumatin-like proteins (TLPs) in plants play a crucial role in combating stress, and they have been proven to possess antifungal properties. However, the role of TLPs in pathogens has not been reported. We identified a effector protein, Pt9029, which contained a Thaumatin domain in Puccinia triticina (Pt), possessing a chloroplast transit peptide and localized in the chloroplasts.
View Article and Find Full Text PDFInt J Mol Sci
May 2024
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Thaumatin-like proteins (TLPs) in plants are involved in diverse biotic and abiotic stresses, including antifungal activity, low temperature, drought, and high salinity. However, the roles of the genes are rarely reported in early flowering. Here, the gene family was identified in .
View Article and Find Full Text PDFPlant Reprod
September 2024
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Two pollen-preferential thaumatin-like proteins show both common and distinctive expression profiles. Precocious expression of one of them drastically disturbs timely deposition and dissolution of callose during microsporogenesis, leading to microspore death. Thaumatin-like proteins (TLPs), members of the pathogenesis-related protein family 5 (PR-5), are involved in plant defenses against biotic and abiotic stresses through antifungal activity and enhanced tolerance.
View Article and Find Full Text PDFInt J Biol Macromol
December 2023
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, Bloco 907, Fortaleza, Ceará CEP 60451-970, Brazil. Electronic address:
Although latex fluids are found in >20,000 plant species, the biochemical composition and biological function of their proteins are still poorly explored. Thus, this work aimed to conduct a proteomic analysis of Cryptostegia grandiflora latex (CgLP) for subsequent purification and characterization of an antifungal protein. After 2D-SDS-PAGE and mass spectrometry, 27 proteins were identified in CgLP, including a polygalacturonase inhibitor, cysteine peptidases, pathogenesis-related proteins (PR-4), and osmotins.
View Article and Find Full Text PDFPeerJ
January 2023
The Institute of Horticulture, ZheJiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
Background: , a soil-borne fungal pathogen, causes significant yield losses in many plants, including cultivated strawberry (, 2n = 8x = 56). Thaumatin-like proteins (TLPs) are a large and complex family of proteins that play a vital role in plant host defense and other physiological processes.
Methods: To enhance our understanding of the antifungal activity of TLPs (), we investigated the genome-wide identification of gene families and their expression patterns in plants upon pathogen infection.
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