AI Article Synopsis
- The liver has two distinct zones, periportal and perivenous, with hepatocytes showing significant differences in enzyme levels and activities.
- Researchers isolated these cell types from mice to conduct microarray analysis, identifying 198 genes with at least a 2-fold difference in expression between them.
- Key findings include the identification of genes involved in various metabolic processes and confirmation of metabolic zonation in the liver, enhancing the understanding of transcriptional regulation.
Article Abstract
Hepatocytes located in the periportal and perivenous zones of the liver lobule show remarkable differences in the levels and activities of various enzymes and other proteins. To analyze global gene expression patterns of periportal and perivenous hepatocytes, enriched populations of the two cell types were isolated by combined collagenase/digitonin perfusion from mouse liver and used for microarray analysis. In total, 198 genes and expressed sequences were identified that demonstrated a >/= 2-fold difference in expression between hepatocytes from the two different zones of the liver. A subset of 20 genes was additionally analyzed by real-time RT-PCR, validating the results obtained by the microarray analysis. Several of the differentially expressed genes encoded key enzymes of intermediary metabolism, including those involved in glycolysis and gluconeogenesis, fatty acid degradation, cholesterol and bile acid metabolism, amino acid degradation and ammonia utilization. In addition, several enzymes of phase I and phase II of xenobiotic metabolism were differentially expressed in periportal and perivenous hepatocytes. Our results confirm previous findings on metabolic zonation in liver, and extend our knowledge of the regulatory mechanisms at the transcriptional level.
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| http://dx.doi.org/10.1111/j.1742-4658.2006.05503.x | DOI Listing |
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