AI Article Synopsis
- The genetic stability assessment is crucial for ensuring the quality of live anti-bacteria vaccines, such as the Brucella melitensis Rev 1 vaccine for brucellosis in small ruminants.
- Thirty-six vaccine strains of B. melitensis Rev 1 were analyzed using multiple-locus variable-number tandem repeat (VNTR) analysis, showing a high level of genetic homogeneity among them.
- The study suggests that MLVA could serve as an important method for quality control in the production of live anti-bacterial vaccines globally.
Article Abstract
The assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. Live attenuated Brucella melitensis Rev 1 is the most effective vaccine against brucellosis in small ruminants. Thirty-six B. melitensis Rev 1 vaccine strains isolated from human or animal sources from different geographic regions, from different commercial batches or laboratory collections were typed by the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) recently described for Brucella spp. Our results demonstrated that B. melitensis Rev 1 group as assayed by MLVA is genetically very homogeneous. We believe that MLVA methodology could be an essential assay to guarantee the quality and stability of live anti-bacterial vaccines being produced worldwide and can be included as in vitro control.
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| http://dx.doi.org/10.1016/j.vaccine.2006.09.063 | DOI Listing |
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