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A method is described for the preparation of sera highly active in reactions with Group A Streptococcus lipoteichoic acid. Ultrasound-destroyed biomass of Streptomyces levoris K-3053 strain is used as the antigen. This strain was isolated in studies of soil Streptomyces; it contains sufficient amounts of nonglycosylated glycerolteichoic acid of the first-third types, that is identical in structure to polyglycerophosphate skeleton of Group A Streptococcus lipoteichoic acid.

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