Objective: To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines.
Method: Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones.
Result: The MS medium with 0.5-1.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg x L(-1) IAA and 3 mg x L(-1) BA was suitable for the induction of calli. MS medium with 1.0 mg x L(-1) IAA and 2.0-3.0 mg x L(-1) BA was suitable for the subculture of calli. MS medium with 2.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the differentiation of calli.
Conclusion: Aseptic seeding of G. stramines can be quickly propagated by shoot culture.
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