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[Determination of fraxinellone in root bark of Dictamnus dasycarpus by RP-HPLC]. | LitMetric

[Determination of fraxinellone in root bark of Dictamnus dasycarpus by RP-HPLC].

Zhongguo Zhong Yao Za Zhi

Department of Chemistry & Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721007, China.

Published: June 2006

Objective: To develop a RP-HPLC method for determination of fraxinellone in Dictamnus dasycarpus.

Method: RP-HPLC conditions were as follows: AT-LICHROM C18(4.6 mm x 250 mm) column, MeOH-H2O (70:30) as a mobile phase, detection wavelength 240 nm, column temperature 25 degrees C, Flow velocity 0.5 mL x min(-1).

Result: Linear relationship is very good (r = 0.9999) in 0.018-0.18 mg x mL(-1). The average recovery is 98.9%, RSD is 1.2% (n = 4).

Conclusion: The method is simple and repeatable and can be used for quantitative analysis of fraxinellone in root bark of D. dasycarpus.

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