New dissymmetrical neutral-cationic or anionic-cationic alpha,omega-diamido bolaamphiphiles have been synthesized in which the polar headgroups are derived from alginate and glycine betaine and which exhibit monolayer lipid membrane vesicles, large lamellae and rods.
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http://dx.doi.org/10.1039/b607368a | DOI Listing |
Unlabelled: Cytoplasmic proteins must recruit to membranes to function in processes such as endocytosis and cell division. Many of these proteins recognize not only the chemical structure of the membrane lipids, but the curvature of the surface, binding more strongly to more highly curved surfaces, or 'curvature sensing'. Curvature sensing by amphipathic helices is known to vary with membrane bending rigidity, but changes to lipid composition can simultaneously alter membrane thickness, spontaneous curvature, and leaflet symmetry, thus far preventing a systematic characterization of lipid composition on such curvature sensing through either experiment or simulation.
View Article and Find Full Text PDFSci Rep
January 2025
University of Novi Sad, BioSense Institute, Dr Zorana Djindjica 1, Novi Sad, 21000, Serbia.
Although various sensors specifically developed for target analytes are available, affordable biosensing solutions with broad applicability are limited. In this study, a cost-effective biosensor for detecting human epidermal growth factor receptor 2 (HER2) was developed using custom-made gold leaf electrodes (GLEs). A novel strategy for antibody immobilization on a gold surface, for the first time mediated by protein L and HER2-specific antibody trastuzumab, was examined using commercial screen-printed gold electrodes and GLEs.
View Article and Find Full Text PDFPhys Chem Chem Phys
January 2025
Department of Physics, School of Natural Sciences, Shiv Nadar Institution of Eminence, NH91, Tehsil Dadri, G. B. Nagar, Uttar Pradesh 201314, India.
Graphene and its derivatives, such as graphene oxide (GO) and reduced graphene oxide (rGO), have propelled advancements in biosensor research owing to their unique physicochemical and electronic characteristics. To ensure their safe and effective utilization in biological environments, it is crucial to understand how these graphene-based nanomaterials (GNMs) interact with a biological milieu. The present study depicts GNM-induced structural changes in a self-assembled phospholipid monolayer formed at an air-water interface that can be considered to represent one of the leaflets of a cellular membrane.
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December 2024
Technological University Dublin, Institute of Polymers, Kevin Street, Dublin 8, Dublin, IRELAND.
Donor-acceptor BODIPY dyads, functionalized at the 2 and 6 positions with benzyl ester (BDP-DE) or carboxylic acid (BDP-DA) groups, were synthesized and characterized for their optoelectronic properties. The introduction of carbonyl groups increased the reduction potential of the BODIPY core by 0.15-0.
View Article and Find Full Text PDFEur J Pharm Biopharm
December 2024
School of Pharmaceutical Sciences, Beijing Advanced Innovation Center for Structural Biology, and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, PR China. Electronic address:
Oral delivery of peptide drugs remains one of the most formidable challenges in the frontier of pharmaceutical research. Peptide drugs typically suffer from exceptionally low oral bioavailability, primarily attributed to rigorous enzymatic degradation within the gastrointestinal (GI) tract, limited ability to traverse the enterocyte barrier, and significant first-pass hepatic metabolism. Absorption of peptide drugs via the lymphatic route could potentially bypass intracellular lysosome degradation and hepatic first-pass metabolism.
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