Objective: To explore the tk gene mutation spectrum in spontaneous and induced WTK1 mutants.
Methods: After exposure to methyl methanesulfonate (MMS), mitomycin (MMC) or sodiam azide (NaN3), spontaneously-arising and induced mutants of WTK1 cell line were selected. The spectrum and hotspot of tk gene mutation were analyzed by multiplex PCR.
Results: Three chemicals used in this study induced tk mutation frequency (MF) to increase significantly in a dose-dependent manner; most of the analyzed mutants had lost exon 4 and exon 5-7.
Conclusion: Three chemicals have mutagenic effect on WTK1 cell line, and obvious hotspot exists in tk gene mutation.
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