[Effect of lenti-mCCL20 on the growth of mouse tumor].

Objects: We aim at inducing a potent antitumor immune response via CCL20 expressing in situ tumor.

Methods: We constructed a recombinant lentivirus encoding mouse CCL20 cDNA and transduced the mouse mesenchymal stem cells (mMSCs) in vitro, and then selected the transfected cells to get a stable mixed mCCL20-expressing pool (named as lenti-mCCL20-mMSCs) with blasticidin. By the same means, we produced another pool named as lenti-null-mMSCs. The CT26 was mixed with lenti-mCCL20-mMSC and inoculated sc into the left hinder back of BALB/c mouse. And also the CT26 was alone, or mixed with lenti-null-mMSCs or parent mMSCs, and then inoculated sc into BALB/c mice serving as controls.

Results: We got a lenti-mCCL20-mMSCs expression construct. It was shown that mCCL20 increased intratumoral lymphocytes infiltration and facilitated tumor growth in syngeneic murine tumor model.

Conclusions: CCL20 expressing in situ tumor enhances intratumoral lymphocytes infiltration but facilitates tumor growth. However, the mechanism involved remains to be further elucidated.