AI Article Synopsis
- The growth and development of plants rely heavily on the specific expression of genes in different organ and cell types, but it's often challenging to gather enough targeted cells for analysis.
- Laser capture microdissection (LCM) is an innovative technique that enables researchers to precisely collect specific cells or even single cells, addressing the issue of tissue heterogeneity in molecular studies.
- The paper discusses the principles behind LCM, summarizes its applications in gene expression analysis, proteomics, and plant-microbe interactions, and suggests future directions for its use in plant research.
Article Abstract
To a large degree, the growth and development of plants depend on the organ and/or cell type-specific expression of genes. However, it can be difficult to obtain sufficient number of specific cells from any developmental stage to analyze expression of genes. Laser capture microdissection (LCM) is a novel technique that allows us to collect pure targeted cell subgroup or even a single cell quickly and precisely, thus the problem of tissue heterogeneity in molecular analysis can be resolved successfully. In this paper, the principles of LCM were introduced and the application of LCM in plant was summarized in gene expression analysis, proteomics and plant-microbe interaction. Meanwhile, possible directions of LCM in plant were put forward.
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| http://dx.doi.org/10.1360/yc-006-1325 | DOI Listing |
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