Fluorescence response upon hybridization of perylene labeled oligonucleotide probes depends on the microenvironment experienced by the perylene fluorophore. In mismatched duplex ((Per)U-C), enhanced fluorescence was observed while in matched duplex ((Per)U-A) fluorescence intensity decreased considerably. This observation will be a promising research effort in giving rise to a new powerful tool in detection of SNP.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bmcl.2006.09.039 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of Cholic Acid Salt and Its Mixed Micelles on the Morphology of Giant Unilamellar Vesicles (GUV).
J Oleo Sci
January 2025
Faculty of Science and Technology, Tokyo University of Science.
Article Synopsis
- Bile salts act as biosurfactants in the gastrointestinal tract, helping to emulsify and absorb fat-soluble nutrients and drugs.
- The study utilized giant unilamellar vesicles (GUVs) to investigate the permeation behavior of bile salts and their mixed micelles, using sodium cholate (NaC) and various lipophilic substances.
- Findings showed that below the critical micelle concentration (CMC), NaC causes endocytic changes in GUVs, while above the CMC, mixed micelles interact with the membrane differently, forming aggregates that migrate into the GUV, with variations observed depending on the type of lipophilic component used.
Application of a Near-Infrared Fluorescence Probe Based on Perylene Bisimide in the Detection of Heparin.
Langmuir
December 2024
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.
Heparin is widely used to treat thrombosis because it is an effective anticoagulant. However, excessive use of heparin can lead to an increased risk of bleeding, which makes the quantitative detection of heparin very important. An amphiphilic perylene bisimide molecule (denoted PBI-9) was developed, which presented a near-infrared emission peak at 730 nm when it was aggregated.
View Article and Find Full Text PDFS/Se-Annulated Star-Shaped Perylene Diimides (PDIs) with Large Two-Photon Absorption (TPA) Cross-Section in NIR-I Region.
Chemistry
January 2025
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Materials and Clean Energy, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, China.
Article Synopsis
- * Perylene diimides (PDIs) are noted for their strong thermal, optical, and chemical stability as n-type organic semiconductors, but they usually have low δ values in the near-infrared region (NIR-I), limiting their effectiveness.
- * Two new star-shaped PDI fluorophores, PDI-S and PDI-Se, were developed, with PDI-S achieving an impressive δ of 3775 GM, among the highest for PDIs in the NIR-I region.
Biosensor development for diabetes diagnosis: Determining relevant miRNA using a newly developed N-annulated perylene fluorescent dye.
Talanta
January 2025
Institute of Analytical Chemistry, Zhejiang University 310058, China. Electronic address:
MicroRNAs (miRNAs) have emerged as essential biomarkers for disease diagnosis, and several techniques are available to determine type 2 diabetes (T2D) relevant miRNAs. However, detecting circulating miRNAs can be challenging due to their small size, low abundance, and high sequence similarity, often requiring sensitive detection approaches combined with additional amplification processes. Laser-induced fluorescence (LIF) is a classic analytical method suitable for sensitively detecting trace amounts of nucleotide acid.
View Article and Find Full Text PDFEnhancing Phototoxicity in BODIPY-Perylene Charge Transfer Dyads by Combined Iodination and Mesylation.
Chemistry
December 2024
School of Chemical Sciences, National Centre for Sensor Research, Dublin City University, Dublin, Ireland.
The uptake and phototoxicity of a family of BODIPY-perylene charge transfer dyads are compared in live cancer and non-cancer cell lines to evaluate their performance in imaging and photodynamic therapy (PDT). The impact of iodination and mesylation of the meso position of the compounds on their optical properties, cell uptake and toxicity are compared. Notably, across all derivatives the probes were minimally dark toxic up to 50 μM, (the maximum concentration tested), but exhibited outstanding phototoxicity with nanomolar IC values and impressive phototoxic indices (PI, ratio of dark IC to light IC), with best performance for the mesylated iodinated derivative MB2PI, which had a PI of >218 and >8.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!