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Development of lateral flow immunochromatographic assay with Anti-Pythium insidiosum antibodies for point-of-care testing of vascular pythiosis.
Sci Rep
January 2025
Center of Excellence for Antimicrobial Resistance and Stewardship, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
The pathogenic oomycete Pythium insidiosum causes a fatal infectious illness known as pythiosis, impacting humans and certain animals in numerous countries in the tropics and subtropics. Delayed diagnosis is a primary factor contributing to the heightened morbidity and mortality associated with the disease. Several new serodiagnostic methods have been developed to improve the identification of pythiosis.
View Article and Find Full Text PDFPathCrisp: an innovative molecular diagnostic tool for early detection of NDM-resistant infections.
Sci Rep
January 2025
CrisprBits Private Limited, 3rd Floor, Plot No.-3, F-301, Ashish Complex, LSC, New Rajdhani Enclave, East Delhi, Delhi, 110092, India.
Article Synopsis
- The study focuses on developing a rapid and accurate molecular detection system, called the PathCrisp assay, to identify infections and antibiotic resistance directly from culture samples.
- The PathCrisp assay uses a combination of loop-mediated isothermal amplification and CRISPR-based detection, showing high sensitivity and specificity, particularly in detecting the New Delhi metallo-beta-lactamase (NDM) gene in clinical samples.
- This novel assay provides results in about 2 hours without the need for complex equipment or extensive DNA purification, aiming to improve antibiotic treatment decisions in various healthcare settings.
A simple improved method for extracting DNA from ethanol-preserved hard ticks and its applications.
Infect Genet Evol
December 2024
Division of Vector Biology and Control, Indian Council of Medical Research - Vector Control Research Centre (ICMR-VCRC), Puducherry 605006, India. Electronic address:
Article Synopsis
- Hard tick exoskeletons make DNA extraction difficult, prompting researchers to test a modified method for extracting DNA from ethanol-preserved ticks for genetic studies.
- The new method was compared to three commercial kits and showed similar DNA concentration and purity across different life stages of ticks.
- The extracted DNA was used for PCR amplification of phylogenetic markers to analyze Amblyomma integrum, a potential disease vector, demonstrating a cost-effective approach that can aid genetic research in low-resource settings.
Comparison of Plant Genomic DNA Extraction Kits Using the Proofman-LMTIA Amplification Assay.
Phytochem Anal
December 2024
School of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan, China.
Introduction: The extraction of DNA is the basis of molecular biology research. The quality of the extracted DNA is one of the key factors for the success of molecular biology experiments.
Objective: To select a suitable DNA extraction method for Chinese medicinal herbs and seeds.
A comparison of routine [Ga]Ga-PSMA-11 preparation using Locametz and Illuccix kits.
EJNMMI Radiopharm Chem
December 2024
Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI, 48109, USA.
Background: Approval of Locametz and Illuccix kits for the manufacture of [Ga]Ga-PSMA-11 (gallium Ga68 gozetotide), a PET imaging agent for prostate cancer, as well as the corresponding therapeutic ([Lu]Lu-PSMA-617 Pluvicto), has led to a rapid increase in demand for [Ga]Ga-PSMA-11 PET imaging. Radiopharmaceutical manufacturers, using Ge/Ga generators, may decide to adopt Locametz and/or Illuccix kits, which requires a comparison to select the most suitable kit for day-to-day use. The objective of this article is to compare both kits and provide guidance for selecting one for routine use, as well as evaluate labeling consistency of both kits during routine production.
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