[hPARP1 genetic polymorphism in southern Chinese Han and Miao populations].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi

School of Public Health, Guangdong Medical College, Zhanjian, Guangdong, 524023 PR China.

Published: October 2006

Objective: To study hPARP1 genetic polymorphism in southern Chinese Han and Miao populations.

Methods: Blood samples from 187 and 210 southern healthy Han and Miao populations were collected. The mutations of exons 12,13,16 and 17 of hPARP1 gene were investigated by PCR-single-strand conformation polymorphism(SSCP).

Results: Fragments of 253 bp,313 bp,175 bp,362 bp within exons 12,13,16 and 17 respectively of hPARP1 gene were amplified by multiple PCR. An SSCP variant in exons 12,13,16 and 17 of PARP1 gene in 187 healthy Han and 210 healthy Miao individuals was identified. Seven single-base substitutions compared with the sequence of PARP1 gene were identified: a T to C transition in exon 12 (Phe548Ser), a G to T transition in exon 13 (Ala683Ser), a G to T transition in exon 16 (Asp798Tyr), and a A to G transition in exon 17 (His808Arg).

Conclusion: There were polymorphism sites in exons 12,13,16,17 of hPARP1 gene in southern Chinese Han and Miao populations; these results may be useful for the establishment of PARP1 genotyping, and these newly described PARP1 alleles would be advantageous indicators for population studies.

Download full-text PDF

Source

Publication Analysis

Top Keywords

han miao
16
transition exon
16
southern chinese
12
chinese han
12
exons 121316
12
hparp1 gene
12
genetic polymorphism
8
polymorphism southern
8
healthy han
8
miao populations
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!