Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1610320 | PMC |
http://dx.doi.org/10.1128/AEM.01446-06 | DOI Listing |
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