Establishment of a gene expression system in Ochrobactrum anthropi.

Appl Environ Microbiol

Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic and State University, Blacksburg, VA 24060, USA.

Published: October 2006

Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1610320PMC
http://dx.doi.org/10.1128/AEM.01446-06DOI Listing

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