The indirect fluorescent antibody test (IFAT) revealed seropositivity to Coxiella burnetii in aged non-laying chickens in poultry farms in a prefecture in the central part of Japan. Seropositivity was 7%, and antibody titers ranged from 16 to 64. No DNA fragment specific for C. burnetii was detected in the chickens by nested-PCR. The prevalence of C. burnetii infection in a prefecture of Japan in which poultry farming prospers was 7%.
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