In vitro fungistatic and fungicidal activities of silver sulfadiazine and natamycin on pathogenic fungi isolated from horses with keratomycosis.

Am J Vet Res

Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.

Published: October 2006

AI Article Synopsis

  • The study aimed to assess the antifungal effectiveness of silver sulfadiazine (SSD) and natamycin against filamentous fungi found in horses' eyes suffering from keratomycosis.
  • Out of the isolated 17 fungal strains, SSD showed a range of effectiveness, with MIC50 at 4 microg/mL and MFC50 at 8 microg/mL, indicating a good level of antifungal activity, while natamycin was less effective with MIC50 at 512 microg/mL.
  • The findings suggest that SSD could be a viable treatment option for equine keratomycosis due to its potent antifungal properties against the isolated fungi.

Article Abstract

Objective: To evaluate the in vitro antifungal properties of silver sulfadiazine (SSD) and natamycin against filamentous fungi isolated from eyes of horses with keratomycosis.

Sample Population: Filamentous fungal isolates obtained from eyes of keratomycosis-affected horses.

Procedures: Fungal culture of ocular samples yielded 6 Fusarium spp; 7 Aspergillus spp; and 1 isolate each of Curvularia, Scopulariopsis, Penicillium, and Chrysosporium. For each fungal isolate, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SSD and natamycin were determined.

Results: For all 17 fungal isolates, SSD MIC distribution ranged from < or = 1 to > 64 microg/mL; MIC50 and MIC90 (MICs at which 50% and 90% of organisms were inhibited) were 4 and 32 microg/mL, respectively. The SSD MFC distribution for all isolates was < or = 1 to > 64 microg/mL; MFC50 and MFC90 (MFCs at which 50% and 90% of organisms were killed) were 8 and > 64 microg/mL, respectively. For all fungal isolates, natamycin MIC distribution ranged from 256 to > 1,000 microg/mL; MIC50 and MIC90 were 512 and > 1,000 microg/mL, respectively. The natamycin MFC distribution for all isolates ranged from 512 to > 1,000 microg/mL; MFC(50) and MFC(90) were each > 1,000 microg/mL.

Conclusions And Clinical Relevance: These in vitro data suggest that SSD is fungicidal against the fungal isolates that were obtained from eyes of horses with keratomycosis and that natamycin is fungicidal against some of the isolates at the drug concentrations evaluated. Silver sulfadiazine may be a therapeutic option for equine keratomycosis.

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Source
http://dx.doi.org/10.2460/ajvr.67.10.1788DOI Listing

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