Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.trstmh.2006.06.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Understanding American tegumentary leishmaniasis in urban Montes Claros, Brazil: insights from clinical, immunological and therapeutic investigations.
Parasitology
November 2024
Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, USA.
The challenge of American tegumentary leishmaniasis (ATL) continues in Brazil, presenting a persistent public health issue despite initiatives aimed at public outreach, vector control and health education. To gain a deeper understanding of this disease, a study was conducted in an endemic region located in the northern region of the state of Minas Gerais, Brazil. The study monitored 30 resident patients diagnosed with ATL, using serum samples from 6 healthy individuals as controls.
View Article and Find Full Text PDFTreatment of Refractory Mucosal Leishmaniasis Is Associated with Parasite Overexpression of HSP70 and ATPase and Reduced Host Hydrogen Peroxide Production (Brief Report).
Biomedicines
September 2024
Post-Graduation Program in Clinical Medicine (PPGCM), Faculty of Medicine (FM), Campus Universitário Darcy Ribeiro, University of Brasília (UnB), UnB Área 1-Asa Norte, Brasilia 70910-900, DF, Brazil.
Background: Mucosal leishmaniasis (ML) is a deforming type of American Tegumentary Leishmaniasis caused by () that frequently does not respond to treatment. Despite its relapsing clinical course, few parasites are usually found in mucosal lesions. Host and parasite factors may be responsible for this paradox in the pathogenesis of the disease, allowing for both a low parasite burden and the inability of the host to clear and eliminate the disease.
View Article and Find Full Text PDFDiagnostic performance of Leishmania braziliensis and Leishmania peruviana antigens in the immunoblot method for the detection of american tegumentary leishmaniasis.
Rev Peru Med Exp Salud Publica
October 2024
Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú.
Article Synopsis
- The study investigates the immunogenic properties of soluble and excretion/secretion antigens from Leishmania braziliensis and Leishmania peruviana to identify potential diagnostic proteins for American Tegumentary Leishmaniasis (ATL).
- Key findings reveal that the soluble antigen of Leishmania braziliensis has high sensitivity (87.7%) and perfect specificity (100%) for ATL detection, while Leishmania peruviana shows slightly better sensitivity (92.3%) and good specificity (95.7%).
- The research suggests that using immunoblot techniques can enhance the diagnostic accuracy for ATL, particularly in challenging cases, and emphasizes the need to further characterize the identified immunogenic regions for developing effective recombinant and synthetic proteins
Senescence-related genes are associated with the immunopathology signature of American Tegumentary Leishmaniasis lesions and may predict progression to mucosal leishmaniasis.
Clin Exp Immunol
October 2024
Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo, Brazil.
The American Tegumentary Leishmaniasis (ATL) is caused by protozoans of the genus Leishmania and varies from mild localized cutaneous leishmaniasis (LCL) form to more severe manifestations such as the diffuse cutaneous leishmaniasis (DCL) form and the mucosal leishmaniasis (ML) form. Previously, we demonstrated the accumulation of senescent cells in skin lesions of patients with LCL. Moreover, lesional transcriptomic analyses revealed a robust co-induction of senescence and pro-inflammatory gene signatures, highlighting the critical role of senescent T cells in orchestrating pathology.
View Article and Find Full Text PDFSpectrum of bacterial pathogens in inflammatory and noninflammatory cutaneous ulcers of American tegumentary leishmaniasis.
Ther Adv Infect Dis
September 2024
Temerty Faculty of Medicine, University of Toronto, ON, Canada.
Background: Cutaneous leishmaniasis (CL) ulcers exhibiting an inflammatory phenotype, characterized by purulent exudate, erythema, pain, and/or lymphatic involvement, are empirically treated with antibiotics.
Objective: The spectrum of bacteria present in localized versus inflammatory phenotypes of CL is elucidated herein.
Methods: Filter paper lesion impressions (FPLIs) from 39 patients with CL (19 inflammatory and 20 noninflammatory ulcers) were evaluated via real-time polymerase chain reaction (qPCR) and end-point PCR targeting: , , , spp.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!