Enterococci for which the minimum inhibitory concentration (MIC) of vancomycin was >/=8 mg/l were isolated from meat, feces, and raw milk samples collected in Korea from March to November 2003. Among the 243 vancomycin-resistant enterococci (VRE) that were identified the vanA vancomycin resistance gene was carried by 51 Enterococcus faecium and one Enterococcus sp., vanC1 was carried by 151 Enterococcus gallinarum, vanC2 was carried by 39 Enterococcus casseliflavus, and one Enterococcus sp. carried no van genes. Of the isolated enterococci carrying vanA, 4% were found to be highly resistant to gentamicin and 11% were resistant to ampicillin. Further genotyping of the E. faecium isolates carrying vanA using pulsed-field gel electrophoresis (PFGE) revealed extensive heterogeneity. The vancomycin resistance transferability test revealed that only two of the 52 enterococci carrying the vanA gene were able to transfer vancomycin resistance to other enterococci. The VRE were recovered from various animal sources with a particularly high prevalence of E. faecium carrying the vanA gene being found in poultry meat.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijfoodmicro.2006.07.023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Vancomycin-resistant Staphylococcus aureus endangers Egyptian dairy herds.
Sci Rep
December 2024
Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, SAR, China.
The emergence of pandrug-resistant (PDR) and extensive drug-resistant (XDR) methicillin-resistant and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) isolates from bovine milk samples along with biofilm formation ability and harboring various virulence genes complicates the treatment of bovine mastitis and highlights the serious threat to public health. This study investigated for the first time the frequency, antimicrobial resistance profiles, biofilm-forming ability, virulence factors, spa and staphylococcal cassette chromosome mec (SCCmec) types of MRSA and VRSA isolated from clinical and subclinical bovine mastitis in Egypt. A total of 808 milk samples were collected from each quarter of 202 dairy animals, including 31 buffaloes and 171 cattle.
View Article and Find Full Text PDFGuardians of resistance and virulence: detection of mec, femA, Van, pvl, hlg and spa genes in methicillin and vancomycin-resistant Staphylococcus aureus from clinical and food samples in Southwestern Nigeria.
BMC Microbiol
November 2024
Multidisciplinary Research Laboratory, Osun State University, Osogbo, 230212, Nigeria.
Background: Staphylococcus aureus strains are highly virulent and associated with an eclectic range of severe nosocomial and community-acquired infections.
Objectives: This study assessed methicillin- and vancomycin-resistant Staphylococcus aureus (MRSA/VRSA) from clinical and ready-to-eat (RTE) food sources, screened for antibiotic resistance; and molecular determinants of antibiotic and virulence genes.
Methods: Altogether, 465 clinical and RTE food samples were analyzed via conventional microbiological techniques and S.
Deciphering the Resistome and Mobiolme of an Avian-Associated ST249 Clone that Acquired Vancomycin Resistance Isolated from Neutropenic Patient in Tunisia.
Microb Drug Resist
December 2024
Laboratory Ward, National Bone Marrow Transplant Center, Tunis, Tunisia.
Article Synopsis
- - The study identified the first vancomycin-resistant Enterococcus (VREfs) strain in Tunisia from an 8-year-old patient with bone marrow issues, using whole-genome sequencing (WGS) after isolating it during a hospital stay.
- - The strain showed significant resistance levels, with minimum inhibitory concentrations of vancomycin >256 mg/L and teicoplanin at 16 mg/L, and was classified as the ST249 clone typically found in avian sources.
- - This report highlights a novel case where an initially vancomycin-susceptible strain acquired resistance via a plasmid that carried a transposon, aided by specific genetic mechanisms related to conjugation and CRISPR interference
Investigation of Various Toxigenic Genes and Antibiotic and Disinfectant Resistance Profiles of Originating from Raw Milk.
Foods
October 2024
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, İstanbul University-Cerrahpaşa, İstanbul 34320, Türkiye.
Article Synopsis
- * The research revealed a high prevalence (89.2%) of antibiotic resistance genes, with the most common being detected in 75% of the samples, and notable resistance to several antibiotics like penicillin and ampicillin.
- * The presence of toxigenic genes and substantial antibiotic resistance in strains found in raw milk poses significant public health and food safety risks.
Cytotoxic Activity of Vancomycin-Resistant Enterococci Isolated from Hospitalised Patients.
Pathogens
September 2024
Department of Microbiology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland.
Vancomycin-resistant enterococci (VRE) are considered one of the main nosocomial pathogens due to their increasing antibiotic resistance and ability to cause life-threatening infections in humans. This study included VRE isolates obtained from various specimens including urine, blood, faeces, wounds, sputum, and oral cavity wash. Of the 37 strains, 30 (81.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!