AI Article Synopsis
- Pannexins are a newly identified family of proteins that may be involved in forming gap junctions.
- Researchers created antibodies against mouse pannexin1 (Panx1) to study its behavior in cells.
- Findings show that while Panx1 is present on the cell surface, it does not appear to form functional gap junctions in certain mammalian cell types, including HeLa, Neuro2a, oligodendrocytes, astrocytes, and hippocampal neurons.
Article Abstract
Pannexins are a newly described family of proteins that may form gap junctions. We made antisera against mouse pannexin1 (Panx1). HeLa cells expressing Panx1 have cell surface labeling, but not gap junction plaques, and do not transfer small fluorescent dyes or neurobiotin in a scrape-loading assay. Neuro2a cells expressing Panx1 are not electrophysiologically coupled. Intracellular Panx1-immunoreactivity, but not gap junction plaques, is seen in cultured oligodendrocytes, astrocytes, and hippocampal neurons. Thus, at least in these mammalian cells lines, Panx1 does not form morphological or functional gap junctions, and it remains to be demonstrated that Panx1 forms gap junction-forming protein in the CNS.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/glia.20435 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!