AI Article Synopsis
- The study introduces a real-time PCR assay that can detect the K65R mutant of HIV-1 reverse transcriptase at a low level (0.5%) in patient plasma samples.
- A total of 73 specimens (53 from treatment-naïve and 20 from treatment-experienced patients) were successfully analyzed using this new method.
- The results from the new PCR method matched those obtained from more traditional, time-consuming sequencing techniques, confirming its reliability.
Article Abstract
We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698293 | PMC |
| http://dx.doi.org/10.1128/JCM.01512-06 | DOI Listing |
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