The 'microheterogeneity model' (R.J. Siezen and R. van Driel, Biochim. Biophys. Acta 295 (1973) 131) and the 'incompetent whole molecule model' (G. Kegeles, Arch. Biochem. Biophys. 180 (1977) 530) for the dissociation of Helix pomatia alpha-hemocyanin whole molecules to half molecules were tested experimentally, using ultracentrifugation and stopped-flow dilution analysis. Results of differential sedimentation experiments followed by stopped flow analysis of separated fractions of 60 S and 100 S molecules were not entirely as predicted by the incompetent model, the agreement depending on the pH and ionic strength of analysis. A considerable amount of stopped-flow dilution response could be attributed to material sedimenting between 60 and 100 S. This material appears to be the main equilibrating fraction, and its amount is considerably larger than that predicted by the microheterogeneity model. Increased hydrostatic pressure was found to enhance this fraction, whereas fixation or low ionic strength reduced or eliminated this fraction. Nonequilibrium components of 30, 50 and 80 S were detected and partially purified by differential sedimentation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0301-4622(84)85011-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An untargeted metabolomics approach to evaluate enzymatically deconjugated steroids and intact steroid conjugates in urine as diagnostic biomarkers for adrenal tumors.
Clin Chem Lab Med
January 2025
Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Objectives: Urinary steroid profiling after hydrolysis of conjugates is an emerging tool to differentiate aggressive adrenocortical carcinomas (ACC) from benign adrenocortical adenomas (ACA). However, the shortcomings of deconjugation are the lack of standardized and fully validated hydrolysis protocols and the loss of information about the originally conjugated form of the steroids. This study aimed to evaluate the quality of the deconjugation process and investigate novel diagnostic biomarkers in urine without enzymatic hydrolysis.
View Article and Find Full Text PDFTargeting Tn Antigen Suppresses Aberrant O-Glycosylation-Elicited Metastasis in Breast Cancer.
J Cell Mol Med
December 2024
Department of Gynecology and Obstetrics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.
Article Synopsis
- - The study investigates the Tn antigen, an abnormal sugar molecule often found in breast cancer, and its relationship to cancer spread (metastasis) and patient outcomes.
- - Researchers discovered that Tn antigen is commonly present in breast cancer tissues, especially in metastatic areas, and its expression is linked to more advanced disease and lower survival rates.
- - Targeting the Tn antigen in breast cancer cells showed promise in reducing their ability to invade and spread, suggesting it could be an effective therapeutic target for treating aggressive breast cancer.
mucin alleviates DSS-induced colitis in mice: Unraveling the cross talk between microbiota and intestinal chemokine.
Heliyon
September 2024
Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt.
Gut microbiota imbalance and alterations in the chemokine-chemokine receptor interactions are pivotal in the initiation and advancement of ulcerative colitis (UC). The current UC treatments are prolonged, exhibit high recurrence rates, and may lead to colorectal cancer. So, this study explores the efficacy of () mucin in preventing DSS-induced UC.
View Article and Find Full Text PDFTransformation of 17β-estradiol to estrone by unknown wild-type enzyme in commercial arylsulfatase from Helix pomatia.
J Chromatogr B Analyt Technol Biomed Life Sci
October 2024
Engineering Laboratory of Low-Carbon Unconventional Water Resources Utilization and Water Quality Assurance, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
This work for the first time reported the complete transformation of 17β-estradiol (E2) to estrone (E1) by unknown wild-type enzyme present in the widely used commercial arylsulfatase derived from Helix pomatia. It was found that acetate could effectively inhibit the unknown enzyme with a half inhibitory concentration (IC) of 140.9 μM, while phosphate and citrate showed no inhibition.
View Article and Find Full Text PDFAccelerated mitochondrial evolution and asymmetric fitness of hybrids contribute to the persistence of Helix thessalica in the Helix pomatia range.
Mol Ecol
August 2024
Leibniz Institute for the Analysis of Biodiversity Change, Zoological Museum, Hamburg, Germany.
Interbreeding and introgression between recently diverged species is common. However, the processes that prevent these species from merging where they co-occur are not well understood. We studied the mechanisms that allowed an isolated group of populations of the snail Helix thessalica to persist within the range of the related Helix pomatia despite high gene flow.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!