Use of DNA arrays for the analysis of outbreak-related strains of Listeria monocytogenes.

Int J Med Microbiol

Laboratoire des Listeria, Centre National de Référence des Listeria, World Health Organisation Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25-28 rue du Dr Roux, F-75724 Paris Cedex 15, France.

Published: December 2006

We evaluated the discriminative power and usefulness of the DNA array technology as compared to DNA macrorestriction pattern analysis for monitoring epidemiologically related clusters of Listeria monocytogenes strains that differ slightly in DNA macrorestriction patterns. We show that this approach allows clarifying the genetic basis of the pattern variations. In the reported outbreak, the differences were due to phage excision, showing the power of this technique in epidemiological studies.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijmm.2006.06.002DOI Listing

Publication Analysis

Top Keywords

listeria monocytogenes
8
dna macrorestriction
8
dna
4
dna arrays
4
arrays analysis
4
analysis outbreak-related
4
outbreak-related strains
4
strains listeria
4
monocytogenes evaluated
4
evaluated discriminative
4

Similar Publications

Listeriolysin O (LLO) is a potent membrane-damaging pore-forming toxin (PFT) secreted by the bacterial pathogen . LLO belongs to the family of cholesterol-dependent cytolysins (CDCs), which specifically target cholesterol-containing cell membranes to form oligomeric pores and induce membrane damage. CDCs, including LLO, harbor designated pore-forming motifs.

View Article and Find Full Text PDF

A variety of phytochemicals from different plants are collected by bees into bee pollen granules. This research focused on evaluating the effects of lactic acid fermentation and enzymatic hydrolysis on the antibacterial activity of bee pollen and its interaction with antibiotics. There is limited knowledge regarding the interactions between treated bee pollen extracts and antibiotics, and this study contributes to the field by providing new insights into the antibacterial activity of pollen subjected to eight distinct treatment methods.

View Article and Find Full Text PDF

Among the various causes of rhomboencephalitis, infection is the most common. However, conventional microbiological methods often yield negative results, making diagnosis challenging and leading to extensive, often inconclusive, diagnostics. Advanced molecular techniques like metagenomic next-generation sequencing (mNGS) offer a powerful and efficient approach to pathogen identification.

View Article and Find Full Text PDF

is one of the most important foodborne pathogens that can cause invasive listeriosis. In this study, the virulence levels of 26 strains of isolated from food and clinical samples in Shanghai, China, between 2020 and 2022 were analyzed. There were significant differences among isolates in terms of their mortality rate in , cytotoxicity to JEG-3 cells, hemolytic activity, and expression of important virulence genes.

View Article and Find Full Text PDF

Over the past decade, foodborne diseases have become a significant public health concern, affecting millions of people globally. Major pathogens like spp., , , and contaminate food and cause several infections.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!