AI Article Synopsis
- The study explored how well human bone material works with mesenchymal stem cells to promote bone growth in mice.
- Mice received different treatments: some with a cell-scaffold combination and others with just bone material for comparison, and assessments were done over 2, 4, and 8 weeks.
- Results showed that the combination led to more bone growth over time, with deproteinated bone showing better bone-forming activity than decalcified bone.
Article Abstract
Objective: To investigate the ectopic osteogenesis potential of human natural bone derived material combined with human bone marrow mesenchymal stem cells (MSCs).
Methods: Cell-scaffold complexes were implanted subcutaneously into the left back of the nude mice, and human natural bone derived material were implanted into the right back as control group. The mice were killed respectively on the postoperative 2 weeks, 4 weeks and 8 weeks. The macroscopic, histopathological, alkaline phosphatase (ALP) activity assay methods were performed to assess the ectopic osteogenesis potential.
Results: The cartilaginous osteogenesis were observed in both deproteinated bone and decalcified bone, and the more new bone tissue formed gradually as the time went by after implantation. ALP activity become stronger followed with the time (P < 0.05), and compared with the decalcified bone, deproteinated bone displayed stronger ALP activity (P < 0.05).
Conclusion: The MSCs and human natural bone derived material can be used as good seed cells and scaffold materials respectively to construct tissue-engineered bone, and as the scaffold material, deproteinated bone has better osteogenesis ability than decalcifed bone.
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