In this paper, we studied the effect of subculture of mother-plants and of preculture of shoot tips of two potato varieties (Dejima, cultivated and STN13, wild) cryopreserved using the droplet-vitrification technique. The subculture conditions (light intensity, aeration and planting density) significantly affected survival of both non-cryopreserved and cryopreserved shoot-tips in both varieties. The subculture duration and the position of the shoot tips on the axis of the in vitro plantlets had a significant (P<0.0001) effect on survival of cryopreserved shoot tips. The optimal subculture duration was 7 and 5 weeks and the optimal size of shoot tips was 1.5-2.0 and 1.0-1.5 mm for var. Dejima and STN13, respectively. Survival of cryopreserved shoot tips was influenced by the sucrose concentration in the preculture medium and the preculture duration. The highest survival of cryopreserved shoot tips was observed after preculture with 0.3 M sucrose for 8 h followed by 0.7 M sucrose for 18 h. These results indicate that the parameters of the subculture of mother-plants and of preculture of shoot tips should be carefully optimized, especially in the case of wild species.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Synthetic Microbial Communities Enhance Pepper Growth and Root Morphology by Regulating Rhizosphere Microbial Communities.
Microorganisms
January 2025
Hunan Provincial Key Laboratory of Agroecological Engineering, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Synthetic microbial community (SynCom) application is efficient in promoting crop yield and soil health. However, few studies have been conducted to enhance pepper growth via modulating rhizosphere microbial communities by SynCom application. This study aimed to investigate how SynCom inoculation at the seedling stage impacts pepper growth by modulating the rhizosphere microbiome using high-throughput sequencing technology.
View Article and Find Full Text PDFBacillus subtilis inoculated in organic compost could improve the root architecture and physiology of soybean under water deficit.
Plant Physiol Biochem
January 2025
Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil.
Bacillus subtilis is known to promote root growth and improve plant physiology, while organic compost enhances soil water retention. This study explored the combined effect of inoculating B. subtilis in organic compost on soybean growth under water deficit.
View Article and Find Full Text PDFRNAi-mediated downregulation of endogenous 4-coumarate: CoA ligase activity in Sorghum bicolor to alter the lignin content, which augmented the carbohydrate content and growth.
Planta
January 2025
Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, India.
This study seeks to improve the biomass extractability of Sorghum bicolor by targeting a critical enzyme, 4CL, through metabolic engineering of the lignin biosynthetic pathway at the post-transcriptional level. Sorghum bicolor L., a significant forage crop, offers a potential source of carbohydrate components for biofuel production.
View Article and Find Full Text PDFMowing facilitated Pb accumulation in bermudagrass by mediating root radial transport.
Plant Physiol Biochem
December 2024
College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address:
Moderate mowing of the shoot is an effective strategy for improving Pb-contaminated soil remediation using bermudagrass. However, the mechanisms by which mowing facilitates Pb uptake and accumulation remain insufficiently understood. Root radial transport is critical in efficient heavy metal uptake and translocation in plants and is influenced by root physiological-biochemical characteristics.
View Article and Find Full Text PDFSeed priming with ascorbic acid and spermidine regulated auxin biosynthesis to promote root growth of rice under drought stress.
Front Plant Sci
December 2024
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Introduction: Drought stress severely hampers seedling growth and root architecture, resulting in yield penalties. Seed priming is a promising approach to tolerate drought stress for stand establishment and root development.
Methods: Here, various seed priming treatments, .
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!