Direct measurement of the force of clot contraction from the plasma in normal subjects and standardization of plasma platelet count, carried out with a retractograph, a newly designed apparatus, have helped assess the platelet retractile activity. Linear relationship between platelet count in the clot from the plasma and the force of the clot contraction, detected in normal subjects, allows one to do without standardization of the platelet count to a preset level when measuring the blood platelet retractile activity in patients.
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PLoS Biol
August 2023
Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
The opportunistic pathogen Pseudomonas aeruginosa causes antibiotic-recalcitrant pneumonia by forming biofilms in the respiratory tract. Despite extensive in vitro experimentation, how P. aeruginosa forms biofilms at the airway mucosa is unresolved.
View Article and Find Full Text PDFEur J Orthop Surg Traumatol
August 2023
Department of Upper Limb Surgery, CHU de Strasbourg, Avenue Molière, 67200, Strasbourg, France.
J Bacteriol
October 2022
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Pseudomonas aeruginosa type IV pili (TFP) are important for twitching motility and biofilm formation. TFP have been implicated in surface sensing, a process whereby surface-engaged cells upregulate the synthesis of the second messenger cAMP to propagate a signaling cascade leading to biofilm initiation and repression of motility. Here, we showed that mutations in PilA impairing proteolytic processing of the prepilin into mature pilin as well as the disruption of essential TFP components, including the PilC platform protein and PilB assembly motor protein, fail to induce surface-dependent cAMP signaling.
View Article and Find Full Text PDFmBio
February 2022
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, USA.
The opportunistic pathogen Pseudomonas aeruginosa relies upon type IV pili (Tfp) for host colonization and virulence. Tfp are retractile surface appendages that promote adherence to host tissue and mediate twitching motility, a form of surface-associated translocation. Tfp are composed of a major structural pilin protein (PilA), several less abundant, fiber-associated pilin-like proteins (FimU, PilV, PilW, PilX, and PilE), and a pilus-associated tip adhesin and surface sensor (PilY1).
View Article and Find Full Text PDFCurr Probl Dermatol
June 2023
Department of Dermatology, The Tattoo Clinic, Bispebjerg University Hospital, Copenhagen, Denmark.
Furrows, lines and wrinkles with distensibility and retractility are a biological need of skin in the service of skin integrity, plasticity and joint motion. The fundamental segmentation and linearity of skin is genetically coded, manifested early in embryonal life and remarkably constant throughout life. The basic pattern established early in embryonal life when facial segments expand and fuse is confounded by overlapping linearities of different backgrounds.
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