As compared with other eukaryotic cells, plants have developed an endoplasmic reticulum (ER)-Golgi interface with very specific structural characteristics. ER to Golgi and Golgi to ER transport appear not to be dependent on the cytoskeleton, and ER export sites have been found closely associated with Golgi bodies to constitute entire mobile units. However, the molecular machinery involved in membrane trafficking seems to be relatively conserved among eukaryotes. Therefore, a challenge for plant scientists is to determine how these molecular machineries work in a different structural and dynamic organization. This review will focus on some aspects of membrane dynamics that involve coat proteins, SNAREs (soluble N-ethylmaleimide-sensitive factor attachment receptor proteins), lipids, and lipid-interacting proteins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jxb/erl135 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An RXLR effector disrupts vesicle trafficking at ER-Golgi interface for Phytophthora capsici pathogenicity.
Mol Cells
December 2024
Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea; Plant Immunity Research Center, College of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:
Article Synopsis
- Phytophthora species secrete effectors to manipulate plant immunity, but the specific mechanisms of their induced necrotic cell death remain unclear.
- The study focuses on an RXLR effector named Pc12 from Phytophthora capsici, which promotes virulence by triggering an unusual ER stress response through interaction with Rab13-2.
- Unlike typical immune responses, Pc12 alters the behavior of Rab13-2, affecting protein localization in the endoplasmic reticulum and inhibiting normal vesicle trafficking, thereby allowing the pathogen to successfully infect the host.
Comparative analysis of 3D and 2D in vitro models of the permanent fish liver cell line RTL-W1: Metabolic capabilities and responses to xenobiotics.
Ecotoxicol Environ Saf
December 2024
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg 405 30, Sweden. Electronic address:
In vitro models based on permanent fish liver cell lines have proven to be versatile tools for examining chemical biotransformation and toxicity. However, their in vivo relevance remains uncertain due to their potentially de-differentiated phenotype. Here, we investigate whether a 3D cell culture environment can restore hepatocyte-like properties of the Rainbow trout liver cell line RTL-W1.
View Article and Find Full Text PDFPaths of Least Resistance: Unconventional Effector Secretion by Fungal and Oomycete Plant Pathogens.
Mol Plant Microbe Interact
September 2024
Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, U.S.A.
Article Synopsis
- Different secretion routes for effectors play a crucial role in the interaction between host plants and pathogens, particularly in eukaryotes, where details are still unclear, hindering disease resistance advancements.
- Fungi and oomycetes utilize both conventional (ER-Golgi) and unconventional (Golgi bypass) pathways for effector secretion, incorporating specific molecular components to facilitate their delivery to the host.
- Effector mRNA translation is influenced by codon usage, particularly AA-ending codons, which affects the efficiency of effector production and pathogen success in invading host plants, highlighting a vital aspect of host-pathogen interactions that requires more research.
Identification and characterization of the COPII vesicle-forming GTPase Sar1 in .
Plant Direct
June 2024
Max-Planck-Institut für Molekulare Pflanzenphysiologie Potsdam Germany.
Eukaryotic cells are highly compartmentalized, requiring elaborate transport mechanisms to facilitate the movement of proteins between membrane-bound compartments. Most proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi apparatus through COPII-mediated vesicular trafficking. Sar1, a small GTPase that facilitates the formation of COPII vesicles, plays a critical role in the early steps of this protein secretory pathway.
View Article and Find Full Text PDFA Hollow TFG Condensate Spatially Compartmentalizes the Early Secretory Pathway.
bioRxiv
March 2024
Department of Cell and Developmental Biology, School of Biological Sciences, University of California San Diego, La Jolla, CA 92093 USA.
In the early secretory pathway, endoplasmic reticulum (ER) and Golgi membranes form a nearly spherical interface. In this ribosome-excluding zone, bidirectional transport of cargo coincides with a spatial segregation of anterograde and retrograde carriers by an unknown mechanism. We show that at physiological conditions, Trk-fused gene (TFG) self-organizes to form a hollow, anisotropic condensate that matches the dimensions of the ER-Golgi interface.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!