AI Article Synopsis
- The Caenorhabditis elegans Gene Knockout Consortium employs a straightforward technique involving chemical mutagenesis of wild-type C. elegans, followed by screening progeny with PCR to find DNA deletions.
- The process includes four main steps: mutagenesis and DNA template preparation, PCR detection of deletions, selecting siblings with the desired traits, and stabilizing the deletions in pure genetic strains.
- Each of these steps is elaborated on in the corresponding chapter.
Article Abstract
The methods used by the Caenorhabditis elegans Gene Knockout Consortium are conceptually simple. One does a chemical mutagenesis of wild-type C. elegans, and then screens the progeny of the mutagenized animals, in small mixed groups, using polymerase chain reaction (PCR) to identify populations with animals where a portion of DNA bounded by the PCR primers has been deleted. Animals from such populations are then selected and grown clonally to recover a pure genetic strain. We categorize the steps needed to do this as follows: (1) mutagenesis and DNA template preparation, (2) PCR detection of deletions, (3) sibling selection, and (4) deletion stabilization. These are discussed in detail in this chapter.
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| http://dx.doi.org/10.1385/1-59745-151-7:51 | DOI Listing |
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