A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain LBA4404 carrying the T-DNA vector of interest. The explants and bacteria are cocultured for 7 to 8 d. Bacteria are removed by rinsing explants in sterile distilled water and by culture on regeneration medium containing the antibiotics carbenicillin or ticarcillin. Transformed callus is selected using kanamycin. Somatic embryos are induced by culture of callus on medium lacking plant growth regulators. As mature cotyledonary stage embryos arise, they are transferred to a fresh medium for shoot development and finally to a medium lacking kanamycin for continued shoot and root development. Transgenic plants can be produced in 9 wk with this protocol. Typically 60 to 80% of inoculated explants produce transgenic plants, and escapes are rare.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1385/1-59745-130-4:301 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-Resolution Single-Pixel Imaging of Spatially Sparse Objects: Real-Time Imaging in the Near-Infrared and Visible Wavelength Ranges Enhanced with Iterative Processing or Deep Learning.
Sensors (Basel)
December 2024
Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.
We demonstrate high-resolution single-pixel imaging (SPI) in the visible and near-infrared wavelength ranges using an SPI framework that incorporates a novel, dedicated sampling scheme and a reconstruction algorithm optimized for the rapid imaging of highly sparse scenes at the native digital micromirror device (DMD) resolution of 1024 × 768. The reconstruction algorithm consists of two stages. In the first stage, the vector of SPI measurements is multiplied by the generalized inverse of the measurement matrix.
View Article and Find Full Text PDFSpatiotemporal Dynamics of SARS-CoV-2 Variants During the First Year of the Pandemic Highlight the Earlier Emergence of the Zeta Variant of Interest in Brazil.
Pathogens
December 2024
Laboratório de Pesquisas em Virologia, Departamento de Doenças Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, SP, Brazil.
During the COVID-19 pandemic, SARS-CoV-2 caused an alarming number of cases and deaths worldwide. Brazil was severely affected from late 2020 onward, especially after the emergence of variants of concern (VOCs) and variants of interest (VOIs). Although much is known about the dynamics and evolution of SARS-CoV-2 VOIs and VOCs in the country, information is still lacking on how the cocirculation of several SARS-CoV-2 lineages, along with the lack of vaccination and low adherence to social isolation measures, shaped the first year of the COVID-19 pandemic in Brazil.
View Article and Find Full Text PDFAdaptive Laboratory Evolution of Flavin Functionality Identifies Dihydrolipoyl Dehydrogenase as One of the Critical Points for the Activity of 7,8-Didemethyl-Riboflavin as a Surrogate for Riboflavin in .
Molecules
December 2024
Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Riboflavin analogs lacking one methyl group (7α or 8α) can still serve as a surrogate for riboflavin in riboflavin-deficient microorganisms or animals. The absence of both methyl groups at once completely abolishes this substitution capability. To elucidate the molecular mechanisms behind this phenomenon, we performed an adaptive laboratory evolution experiment (in triplicate) on an strain auxotrophic for riboflavin.
View Article and Find Full Text PDFHeat Stress Prevention in Construction: A Systematic Review and Meta-Analysis of Risk Factors and Control Strategies.
Int J Environ Res Public Health
December 2024
School of Civil & Environmental Engineering, and Construction Management, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
In hot and humid work environments, construction workers can experience heat stress and heat-related illnesses (HRIs). While several studies have investigated engineering and administrative control methods to prevent certain heat stress risk factors, a comprehensive understanding of all existing risk factors and their corresponding control strategies is still lacking. It is crucial to identify gaps in current control strategies and develop a safety management framework for effective heat stress control by implementing existing measures.
View Article and Find Full Text PDFCharacterization and genomic analysis of Abortusequi phage, vB_SalP_LDDK01, and its biocontrol application in donkey meat.
Front Cell Infect Microbiol
January 2025
College of Agriculture and Biology, Liaocheng University, Liaocheng, Shandong, China.
Abortusequi ( Abortusequi) is the primary cause of abortions in equine animals, and can cause serious foodborne illness. Thus, effective biocontrol strategies are needed to decontaminate and control the emergence of foodborne diseases. In recent years, phages have been used as a new strategy for modulating foodborne pathogens and food safety.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!