[Production and characterization of gold labeled monoclonal antibody against MC-LR].

Objective: Production of the gold labeled monoclonal antibody against MC-LR. Study the simple mechanism of the conjugation by the spectral analysis.

Methods: Production and purification monoclonal antibodies against MC-LR by hybridoma technology. Production the 20nm colloidal gold and label the monoclonal antibody. Study the gold--McAb conjugate by the spectroanalysis.

Results: Monoclonal antibody produced by the hybridoma cells were tested for subtypes and designated as IgG2a for 8C11. The titers of antibody in ascites was 108.The IC50 for MC-LR was around 3ng/ml. The IgG molecule weight was 1.5 x 10(5). The structure of the antibody was altered to more tighter and stabler.

Conclusion: Product the gold labeled monoclonalantibody against MC-LR conjugate successfully and keep the antige-binding determinant.