A selenium-dependent Bacillus sp. is able to grow well up to 3% sodium selenite-containing media. The bacterium completely failed to grow on media devoid of selenium. The presence of selenium in the growth media increased the bacterial contents of proteins, carbohydrates, and lipids. The highest quantities of amino acids were detected at 2% sodium selenite-containing media. The bacterium metabolized selenite into several protein selenoamino acids such as selenomethionine and selenocysteine/selenocystine, as well as nonprotein selenoamino acids, such as selenocystathionine. Several phosphoamino acids were detected in the presence of elevated levels of selenium. The synthesized protein seems not to be affected by the presence of selenium.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/BF02990413 | DOI Listing |
Pharmacol Rep
December 2018
Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warszawa, Poland.
Background: Selenium (Se) is an essential micronutrient for animals and humans used in the prevention or treatment of cancer. Selol is a mixture of selenitetriglycerides, containing Se(IV). It does not exhibit mutagenic activity and is less toxic than inorganic sodium selenite containing Se(IV).
View Article and Find Full Text PDFCurr Microbiol
May 2009
USDA-ARS, Fort Collins, CO 80526-8119, USA.
A Pseudomonas sp. that may be useful in bioremediation projects was isolated from soil. The strain is of potential value because it reduces selenite to elemental red selenium and is unusual in that it was resistant to high concentrations of both selenate and selenite.
View Article and Find Full Text PDFBiofactors
January 2000
Department of Plant Biology, University of California, Berkeley 94720, USA.
When introduced into a chemically defined minimal medium supplemented with 1 mM sodium selenite (79 ppm Se(o)), Bacillus subtilis was found to undergo a series of morphological and biochemical adaptations. The morphological changes included the formation of "round bodies" associated with the detoxification of selenite to elemental selenium. Round bodies observed transiently were not apparent during balanced growth of cells adapted previously to selenite-containing medium.
View Article and Find Full Text PDFFree Radic Biol Med
December 1996
German Institute of Human Nutrition, Potsdam-Rehbrücke, Germany.
Commercially available calf serum did not supply the cultured murine fibroblast cell line L929 with amounts of selenium and alpha-tocopherol sufficient to protect against peroxide damage. Supplementation of the culture medium with 30 microM alpha-tocopherol or 50 nM sodium selenite led to a substantial increase of cellular alpha-tocopherol concentrations from 18 +/- 3.0 to 3179 +/- 93.
View Article and Find Full Text PDFJ Trace Elem Electrolytes Health Dis
September 1991
Laboratoire de Biochimie C, Hospital A. Michallon, Grenoble, France.
Many forms of selenium supplement are commercialized. The purpose of this work was to study the bioavailability of one selenium drug, "Granions de Sélénium", as compared with sodium selenite. Each product was labelled with enriched stable isotopes, and by means of gas chromatography/mass spectrometry (GC/MS), the metabolism of the two forms was followed simultaneously.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!